Author: Jawaid Ahmed Zai, Muhammad Rashid Khan, Zaib-Un-Nisa Mughal, Falak Sher Khan and Noorulain Soomro
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Anti-oxidant and anti-inflammatory activities of phytol were investigated in human PBMCs challenged with the oxidative stress using H2O2. The OH ion released from H2O2 is a potent ROS that burden the cellular antioxidants, results excess of active oxygen radicals and stimulate the synthesis of inflammatory mediators. NFκB activation plays important role in establishing and increasing the inflammatory response as well as production of more ROS. Cells were treated with 100µM H2O2 and various concentration of phytol. The cellular antioxidants and NO were estimated. The expression of inflammatory genes IL6, TNFα, IL1β, COX2 and iNOS was determined using QRTPCR and protein expression for IL6, TNFα, IL1β and PGE2 was studied using ELISA. NFκB activation was studied by estimating IKKβ and IκBα phosphorylation level. Addition of H2O2 to the cultured cells results reduced level of antioxidants SOD, GPx and catalase, while higher level of CAT and NO were observed. Phytol treatment results restoration of these variables in dose dependent manner. Similarly H2O2 treatment results higher expression of IL6, TNFα and IL1β both at mRNA and proteins level. It also has results elevated gene expression for COX2 and iNOs, increased synthesis of PGE2 and activation of NFκB. Phytol co treatment to the cells results significant lowering of the inflammatory mediators and NFκB activation. Understanding the mechanism, Phytol results in its therapeutic effects is a challenge in its recruitment as a potential drug. In the present study, NFκB activation is studied in conjunction with the inflammatory cytokines that can induce its activation, in turn their expression is stimulated by the products of NFκB pathway cyclically. The results of present study shown that phytol can alleviate the H2O2 induced oxidative stress and inflammatory response, and prevent the NFκB from activation.
Phytol, H2O2, PBMCs, Oxidative stress, inflammation, NFκB
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How to cite this article: Jawaid Ahmed Zai, Muhammad Rashid Khan, Zaib-Un-Nisa Mughal, Falak Sher Khan and Noorulain Soomro (2021). Phytol Anti-oxidative and Anti-inflammatory Effects in Hydrogen Peroxide Challenged Human PMBCs Involves NFκB Pathway. International Journal on Emerging Technologies, 12(1): 296–303.
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