This study investigates the issue of antibiotic-resistant bacteria in drinking water from various sources within the Sehore district of Madhya Pradesh, highlighting a critical public health concern. Access to clean water is a vital necessity for human health, yet ensuring its provision remains a challenge in many developing regions. The research employs rapid detection methods to identify antibiotic-resistant bacterial strains. Samples were gathered from taps, borewells, water tanks, and the Narmada River in different villages and were cultivated on Nutrient Agar at 37°C to encourage bacterial growth. Three types of bacterial isolates Pseudomonas sp., Escherichia coli, and Salmonella sp. were previously identified and subjected to antibiotic susceptibility testing using the disc diffusion method. The analysis revealed significant antibiotic resistance among the isolates, with Escherichia coli showing resistance to ampicillin, norfloxacin, and ciprofloxacin. Pseudomonas sp. demonstrated moderate resistance in some instances, while the no Salmonella sp. cases identified remained sensitive to the antibiotics tested. The study emphasizes the need for persistent monitoring of drinking water to detect antibiotic-resistant bacteria, thereby safeguarding public health. It calls for the development of efficient strategies to manage and mitigate waterborne bacterial threats. The evidence presented points to the necessity for enhanced water quality management in the Sehore district, addressing both environmental and public health challenges posed by antibiotic resistance.

Antibiotic resistance, Pseudomonas sp., Escherichia coli, Salmonella sp., Madhya Pradesh

The study highlights the alarming prevalence of antibiotic-resistant bacteria in drinking water sources in the Sehore district. The significant resistance observed among E. coli isolates, along with the presence of resistant Pseudomonas sp., underscores the urgent need for continuous monitoring and effective water quality management strategies. By addressing these challenges, it is possible to safeguard public health and mitigate the impact of antibiotic resistance on community health in Sehore and similar regions.

Shilpa Israni, Rakesh Mehta and Ragini Gothalwal (2025). Assessment of Antibiotic Resistance in Bacterial Isolates from Drinking Water Sources in Sehore District, Madhya Pradesh, International Journal of Theoretical & Applied Sciences, 17(1): 51–55.