Biocontrol Potential of Isolated Native Strain of EPNs from Southern Rajasthan against Plutella xylostella (Linnaeus) (Lepidoptera:Plutellidae)

Author: Ankit Kumar V., S. Ramesh Babu, Vijay Kumar and Bhanu Partap Singh

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Abstract

The diamondback moth, Plutella xylostella L., is a serious pest of cole crops. It has developed resistance to several pesticides, including as Bt toxins and chemical insecticides. An effective substitute for chemical pesticides in the management of the diamondback moth is the use of suitable bio-agents. The purpose of the current study was to control diamondback moths using an entomopathogenic nematode (EPN) strain from southern Rajasthan. To determine the virulence and reproductive capacity of an indigenous population, laboratory investigations were conducted using five dosages of the diamondback moth—50, 100, 200, 400, and 1000 IJs per third instar. The percentage of fatalities was noted 24 and 48 hours following the vaccination. With the highest dose, 600 IJs per larva, the maximum percent mortality (66%) was observed after 48 hours, and the LC50 value was 735 IJs/larva. Overall, the data clearly indicated that P. xylostella is more vulnerable to entomopathogenic nematodes and may serve as a host for their growth in laboratory settings.

Keywords

S. siamkayai, Plutella xylostella, Heterorhabditis, Steinernematidae

Conclusion

The present investigation provides basic knowledge regarding the efficacy of entomopathogenic nematode S. bicornutum against Pluttella xylostella. The results strongly suggested that the diamondback moth, Plutella xylostella is susceptible to the entomopathogenic nematode. A thorough investigation at field level on this aspect to develop S. bicornutum as a pest control agent, is necessary.

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