Accelerated Stability Studies of Mucuna prureins Hydroalcoholic Extract Phytosome Formulation, and Evaluation of its Capsule Dosage Form

Author: Poonam Karekar, Suresh Killedar, Harinath More, Amir Shaikh, Sneha Joshi, Supriya Waghmare, Amruta Walvekar, Rahul Buchade and Sarika Patil

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Abstract

Mucuna prureins extract (MPE) is useful in reducing depression symptoms. However, MPE's transit through biological membranes is restricted due to its large molecular weight and hydrophilic nature. Phytosomes could be promising carriers for improving the oral absorption of such encapsulated extracts. One of the major challenges was to get the desired EE and that was achieved by using soya phosphatidylcholine. In this work optimized batch of Mucuna prureins phytosomes (MPP) was subjected to accelerated stability studies and evaluated at regular time intervals for entrapment efficiency, particle size, polydispersity index, and zeta potential. Further, capsules of MPE and MPP were formulated and evaluated for quality control evaluation tests like weight variation, in vitro disintegration time, and in vitro dissolution testing. The accelerated stability studies revealed that the optimized phytosomeformulation was stable under the stability conditions of temperature and relative humidity. Moreover, MPE and MPP had weight variation, and in vitro disintegration time within permissible limits according to IP. The dissolution profile of MPP was found superior to MPE capsules. The findings demonstrated the ability of Mucuna prureins phytosomes to maintain quality control features under accelerated stability conditions. Furthermore, phytosomes could be given in a capsule dosage form, allowing for a prolonged release of encapsulated extracts with long-term health benefits.

Keywords

Mucuna prureins extract, phytosomes, accelerated stability studies, tablet, evaluation

Conclusion

The standardized extract of Mucuna prureins and phospholipids was used to make the phytosome complex. The optimized batch from the previous results was subjected to accelerated stability studies and the sample was evaluated for different quality control tests. These phytosomes also encapsulated 95.63 % of total phenolics, shielding them from the hostile environment of heat and humidity. The optimized batch of phytosomes was found stable at the end of 6 months from the readings of particle size, zeta potential, and polydispersity index. The phytosome capsules possessed the required quality attributes. However, the MPE and MMP capsules differed in the dissolution profiles. The results highlighted the potential of Mucuna prureins phytosomes to retain the quality control attributes in adverse conditions of accelerated stability conditions. Moreover, phytosomes could be delivered in the form of tablet dosage form, offering sustained release of encapsulated extracts that could lead to long-term health benefits. Thus, the authors of this study propose that phytosome drug delivery systems could be considered attractive options for the delivery of bioactive substances in the future.

References

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How to cite this article

Poonam Karekar, Suresh Killedar, Harinath More, Amir Shaikh, Sneha Joshi, Supriya Waghmare, Amruta Walvekar, Rahul Buchade and Sarika Patil (2023). Accelerated Stability Studies of Mucuna prureins Hydroalcoholic Extract Phytosome Formulation, and Evaluation of its Capsule Dosage Form. Biological Forum – An International Journal, 15(5): 1135-1139.