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An Efficient In Vitro Regeneration Methods for Elite Indica Rice Varieties

Author: Laxmipreeya Behera, Kailash Ch. Samal, Parmeswaran C, Pawan Kumar Agrawal, Manasi Dash, Abinash Mishra, D.P. Mishra, Raj Kumari Bhol and Sanghamitra Samantaray

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Abstract

The findings underscore the importance of selecting appropriate hormonal combinations for maximizing shoot regeneration across different rice varieties. The specific combination of BAP, kinetin, and NAA not only facilitated high regeneration rates but also ensured robust growth and survival of the regenerated shoots. This hormonal synergy is crucial for the efficient propagation of plantlets in vitro, which is a foundational step for various plant breeding and genetic engineering applications. Among the tested varieties, Khandagiri exhibited the highest shoot regeneration and survival rates. This variety showed a remarkable ability to regenerate shoots under the given hormonal conditions, outperforming the other two varieties. The precise shoot regeneration and survival percentages for Khandagiri were at the upper end of the observed range, highlighting its responsiveness to the optimized medium. This superior performance suggests that Khandagiri has a high intrinsic capacity for shoot regeneration when exposed to the combination of BAP, kinetin, and NAA. This makes Khandagiri an excellent candidate for tissue culture applications and genetic transformation efforts, as its high regeneration efficiency can significantly enhance the success rate of these biotechnological processes

Keywords

Rice, Lalat, MTU-1010, Khandagiri, Callusing, Regeneration

Conclusion

The findings underscore the importance of selecting appropriate hormonal combinations for maximizing shoot regeneration across different rice varieties. The specific combination of BAP, kinetin, and NAA not only facilitated high regeneration rates but also ensured robust growth and survival of the regenerated shoots. This hormonal synergy is crucial for the efficient propagation of plantlets in vitro, which is a foundational step for various plant breeding and genetic engineering applications. Among the tested varieties, Khandagiri exhibited the highest shoot regeneration and survival rates. This variety showed a remarkable ability to regenerate shoots under the given hormonal conditions, outperforming the other two varieties. The precise shoot regeneration and survival percentages for Khandagiri were at the upper end of the observed range, highlighting its responsiveness to the optimized medium. This superior performance suggests that Khandagiri has a high intrinsic capacity for shoot regeneration when exposed to the combination of BAP, kinetin, and NAA. This makes Khandagiri an excellent candidate for tissue culture applications and genetic transformation efforts, as its high regeneration efficiency can significantly enhance the success rate of these biotechnological processes

References

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How to cite this article

Laxmipreeya Behera, Kailash Ch. Samal, Parmeswaran C, Pawan Kumar Agrawal, Manasi Dash, Abinash Mishra, D.P. Mishra, Raj Kumari Bhol and Sanghamitra Samantaray (2022). An Efficient In Vitro Regeneration Methods for Elite Indica Rice Varieties. Biological Forum – An International Journal, 14(1): 1838-1841.