This study was designed to investigate the impact of high protein high energy formulation on exercise performance in relation to strength capacity tests. To assess the in-vivo efficacy of the developed product in terms of strength capacity, body weight, muscle, and liver glycogen level, the Wister rats were given the developed food formulations for 28 days. Four formulations namely TS1, TS2, TS3, and TS4 were developed and TS4 exhibited the highest scores for all the sensory attributes, nutritive value, and antioxidant activity and was selected for in-vivo studies. The experimental rats were divided into five groups consisting of six rats in each group namely Control (100% standard rat ration), Group I (100% test diet), Group II (75% test diet + 25% standard rat ration), Group III (50% test diet + 50 % standard rat ration), Group IV (25% test diet + 75% standard rat ration). The highest significant (p<0.05) increase in running time, distance covered, muscle glycogen, and liver glycogen level was found in Group I fed with 100% test diet. The present study provided science-based evidence to support that high protein high energy formulation could be a promising anti-fatigue agent and an ergogenic aid

Strength capacity, nutrition, ergogenic aids, high protein high energy supplementation, motor performance, sports nutrition

It can be concluded that a high protein high energy bar which is rich in protein, essential fatty acids, minerals such as iron, zinc, magnesium and phosphorus and soluble fibres played an important role in physiological protection and performance elevation with strength and endurance exercise of experimental rats. Present study provided substantial evidence that supplementation of multiple blend nutraceutical bar resulted in increase in liver and muscle glycogen storage of experimental rats which contributed to extending the running time and swimming time. High protein high energy bar increased the activity of antioxidant enzymes and anti-fatigue activity by increasing hemoglobin level, liver and muscle glycogen depletion thereby elevating exercise performance. The present study provided science-based evidence to support that high protein high energy bar could be a promising anti- fatigue agent and an ergogenic aid. It can be concluded that multiple blend nutraceutical bar which is rich in protein, essential fatty acids, minerals such as iron, zinc, magnesium and phosphorus and soluble fibres played an important role in physiological protection and performance elevation with strength and endurance exercise of experimental rats. Present study provided substantial evidence that supplementation of multiple blend nutraceutical bar resulted in increase in liver and muscle glycogen storage of experimental rats which contributed to extending the running time and swimming time. Multiple blend nutraceutical bar increased the activity of antioxidant enzymes and anti-fatigue activity by increasing hemoglobin level, liver and muscle glycogen depletion thereby elevating exercise performance. The present study provided science-based evidence to support that multiple blend nutraceutical bars could be a promising anti-fatigue agent and an ergogenic aid

Jyotismita Konwar, Mamoni Das and Jadav Sarma (2025). Antifatigue Effect of High Protein High Energy Supplementation Improves Motor Performance and Glycogen Content in Wistar Rats Subjected to Strength Capacity Test. Biological Forum, 17(4): 01-08