Antifungal Activity of Bacillus spp. against Curvularia lunata causing Grain Discolouration of Rice

Author: R. Yuvarani*, S. Thiruvudainambi, M. Theradimani, C. Vanniarajan4, R. Renuka and S. Brindha Devi

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Abstract

Grain discoloration complex disease of rice is an emerging threat to rice crop all over the world and it acquires particular importance as it results in qualitative loss of harvested crop. The study's objective is to evaluate the effective Bacillus subtilis strain BS3 is at preventing rice grain discoloration. Twenty Bacillus spp. were examined in vitro for their ability to inhibit Curvularia lunata. The most effective isolate was chosen based on the antagonism and validated by 16s rRNA. PCR investigation revealed the antibiotic biosynthetic genes that produce bacillomycin, bacilysin, iturin, surfactin, subtilin, mersacidin, subtilosin, ericin, mycosubtilin, and fengycin in the successful isolates. Among the isolates, BS3 inhibited the pathogen's mycelial development the most (48.8%) and had the most antibiotic genes (6). By using gas chromatography mass spectrometry, secondary metabolites from B. subtilis (BS3) were discovered as Phenylethyl alcohol, Benzene ethanol, 4-hydroxyquinolin

Keywords

Curvularia lunata, Bacillus spp., secondary metabolites, GCMS.