Antioxidant and Antibacterial Evaluation of 2,6-dimethoxy-4-((phenylamino) methyl) Phenol and 2,6-Dimethoxy-4-((4'-nitrophenylimino)methyl) Phenol

Author: Tanvi Sahni*, Sunita Sharma, Diksha Verma, Poonam Sharma and Sukhmannpreet Kaur

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Abstract

Two noval Shiff bases i.e. 2,6-dimethoxy-4-((phenylamino) methyl)phenol (I) and 2,6-Dimethoxy-4-((4ï‚¢-nitrophenylimino)methyl)phenol (II) of syringaldehyde containing aniline as the scaffold were developed in the the lab and evaluated for their property as antioxidant using DPPH assay, FRAP assay, and phosphomolybdate assay. It was found that syringaldehyde the precursor of Schiff bases I and II had better DPPH reducing capacity while compound I was effective in reducing ferric Fe(+3) ions and 4-nitro aniline schiff base compound (II) was most effective in reducing Mo(+5) ions. The anti-microbial evaluation has proceeded for syringaldehyde and their Schiff bases I and II along with standard ampicillin against five bacteria viz Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Klebsiella spp., and Enterobacter spp. Compound II was more effective than standard ampicillin against P. aeruginosa only.

Keywords

Syringaldehyde, aniline, 4-nitroaniline, DPPH, FRAP, Phosphomolybdate, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Klebsiella spp., and Enterobacter spp.

Conclusion

It may be concluded that aniline Schiff bases of syringaldehyde possessed better antibacterial activity than antioxidants. They were more effective in controlling Gram-negative bacterium than Gram-positive bacterium and were found to be more effective than standard ampicillin against P. aeruginosa. There is positive interactions between compounds and bacteria at different concenterations. Syringaldehyde had better radical scavenging activity than Schiff bases in terms of DPPH assay while the reverse trend was found in the reduction of ferric Fe (+3) and Mo (+5). None of the compounds was a better antioxidant than standard ascorbic acid and BHT. Thus, possessed moderate antioxidant potential.

References

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How to cite this article

Sahni, T.; Sharma, S.; Verma, D.; Sharma, P. and Kaur, S. (2022). Antioxidant and Antibacterial Evaluation of 2,6-dimethoxy-4-((phenylamino) methyl) Phenol and 2,6-Dimethoxy-4-((4-nitrophenylimino)methyl) Phenol. Biological Forum – An International