Application of in vitro Biotechnological Tools in Coleus forskohlii: An Important Source of Therapeutic Products

Author: Pooja Patel

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Abstract

The various plant species of Coleus genus are commercially important in the field of medicines, components of food and as ornamental plants. Among them, Coleus forskohlii is known for sole source of forskolin production naturally. Since the initiation of plant tissue culture, it has boosted the improvement of crops through various ways. The present review summarized various systems of in vitro regeneration for commercial production of plantlets as well as establishment of suspension culture and transformation protocol for improving forskolin production in vitro. Traditional regeneration system for this plant are not that much efficient and content of forskolin in each plant differed hence, the application of in vitro regeneration system and suspension culture can be a useful tool to overcome such limitations. Several studies have been carried out to develop a rapid and efficient protocol for regeneration of plantlets as well as suspension culture for Coleus forskohlii. It has been observed that various factors like choice of explants, sterilizing agents, selection of basal medium with appropriate plant growth regulators are important for desirable results. It is also concluded by this review that there is still requirement of more studies for improvement of regeneration efficacy as well application of somaclonal variation and determination of genetic stability in tissue cultured plantlets. More studies should be conducted to establish genetic engineering protocol using direct as well as indirect methods of gene transfers to achieve ample yield of forskolin.

Keywords

Coleus forskohlii, forskolin, Organogenesis, Suspension culture, Callus

Conclusion

This review elaborates on the biotechnological advances made on C. forskohlii upto date. There are several findings on direct and indirect organogenesis. This article addressed the impact of various explant sources, sterilization agents, and plant growth regulators on the micropropagation using direct and indirect regeneration system. Coleus nodal segments were the best source of explants for culture initiation because they tolerated sterilization better than apical shoots, demonstrating the highest survival frequency. There are some reports on somatic embryogenesis and genetic transformation but needs further work to establish robust and rapid protocols.

References

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How to cite this article

Pooja Patel (2023). Application of in vitro Biotechnological Tools in Coleus forskohlii: An Important Source of Therapeutic Products. Biological Forum – An International Journal, 15(5): 1031-1037.