Approach towards Submerge Production, Characterization and Application of Extracellular Alkaline Protease from Pseudomonas aeruginosa YPVC

Author: Vijay Chaudhary, Yogesh Patel*, Kavin Soni, Gaurav Dave, Abidali Bhagat1, Jalpesh Patel, Arvind Parmar and Surabhi Joshi

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Abstract

The trend of utilizing eco friendly products is consistently increasing, which results into replacement of several chemical products with microbial formulations. Alkaline protease is an important microbial metabolite having diverse industrial applications. Therefore, in present study, alkaline protease production was carried out from soil dwelling bacterial cultures. Total 66 bacteria amongst 135 were found to be enzyme producers. The isolate VV19 displayed the highest enzyme production with 309.12 ± 4.1 U/ml activity in 24 h under submerge condition. This isolate was identified as Pseudomonas aeruginosa YPVC (GenBank accession number MN049574). The optimized culture conditions pH 10, temperature 30oC, inoculum size 7% (v/v), xylose 0.3%, KNO3 0.2% and NaCl 0.1% resulted into 2.4 fold increment in the alkaline protease productivity. Partial purification of the enzyme resulted into 7.62 purification and 53.51% enzyme yield after dialysis. The enzyme exhibited superior activity at optim

Keywords

Alkaline protease; enzyme activity, Pseudomonas aeruginosa YPVC; optimization; characterization.