Assessment of Antimicrobial Resistance (AMR) in Aeromonas hydrophila Isolated from Freshwater Rohu (Labeo rohita) in Ganjam District, Odisha

Author: N. Das, S. Dash and B.K. Khuntia

Download PDF

Abstract

Swab samples from the gills of live rohu (Labeo rohita) were collected in TSB from various aquaculture farms and reservoirs in the Ganjam district of Odisha and examined for the presence of Aeromonas hydrophila species. The isolates which had shown growth on RS agar or Cetrimide agar were furthered studied by doing gram staining, biochemical tests and observation of their morphology under compound microscope. Bacteria isolates that tested positive for catalase, oxidase, indole, MR, VP, and produced H2S were identified as potential A. hydrophila. In contrast, isolates that tested positive for oxidase but negative for indole, MR, VP, and did not produce H2S were identified as potential P. aeruginosa species. The antimicrobial resistance (AMR) of these isolates was evaluated using the disk diffusion method with antibiotics such as Chloramphenicol (C), Tetracycline (TE), Ciprofloxacin (CIP), Gentamicin (GEN), and Cefotaxime (CTX). The inhibited zone was measured to govern the sensitivity or resistance of the isolates. A. hydrophila isolates from different regions of the Ganjam district exhibited complete resistance (100%) to Ciprofloxacin (CIP) and Chloramphenicol (C), while showing complete susceptibility (100%) to Gentamicin (GEN), Tetracycline (TE), and Cefotaxime (CTX)

Keywords

A. hydrophila, Biochemical test, Antibiotics, AMR, Rohu

Conclusion

It gives an important message to the society that the fishes contaminated with these bacteria from the respective farm if come in contact with human being these bacteria also show the antibiotic resistance to these particular antibiotics. Some antibiotics are susceptible to these bacteria, so further research may be needed for their use in Aquaculture practices. Again some antibiotics are banned still some fish farm of Ganjam district are using this, so information shall be given to them not to use that. Further AMR study is necessary for other pathogen also in aquafarming sector.

References

-

How to cite this article

N. Das, S. Dash and B.K. Khuntia (2022). Assessment of Antimicrobial Resistance (AMR) in Aeromonas hydrophila Isolated from Freshwater Rohu (Labeo rohita) in Ganjam District, Odisha. Biological Forum – An International Journal, 14(1): 1847-1851