Assessment of different Media on Growth and Sclerotia Formation of Sclerotinia sclerotiorum causing Sclerotinia Rot of Chilli under Laboratory condition

Author: Satyadev Prajapati*, Naresh Kumar, Shailesh Godika, Lalita Lakhran, Shivam Maurya and Sunil Kumar

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Abstract

The efficacy of several solid and liquid media on Sclerotinia sclerotiorun growth (on the 7th day) and sclerotia formation (on the 15th day) was tested in the lab. The Potato Dextrose Agar medium had the highest mycelial growth (90.00 mm) and the most sclerotia formation (28.67), followed by Malt extract agar (76.68 mm and 17.33) and Oat meal medium (59.00 mm and 10.34), and Martins medium had the lowest mycelial growth (11.00 mm) and the fewest sclerotia formation (0.00). After 14 days of inoculation at 25oC, Potato Dextrose broth had the highest dry mycelial weight (170.16 mg) and number of sclerotia formation (21.75) of the five liquid media examined. Following that was Richard's medium (147.98 mg and 15.75). Asthana and Hawker’s medium was recorded least dry mycelial weight and numbers of sclerotia formation.

Keywords

Solid and liquid media, mycelial, sclerotia, Sclerotinia clerotiorun.

Conclusion

Nutrition is critical for improved development and sclerotia formation. Potato Dextrose Agar Medium, followed by Malt Extract Agar Medium, was shown to be the best for maximal growth and sclerotia production after studies on the influence of six different solid media on growth and sclerotia formation. Martin's medium had the least amount of growth and no sclerotia. After 14 days of inoculation, studies on the influence of several liquid media on dry mycelial weight and sclerotia development of Sclerotinia sclerotiorum in vitro revealed that Potato dextrose broth had the highest dry mycelial weight and sclerotia formation, followed by Richards medium. The medium Asthana and Hawker species, on the other hand, had the lowest dry mycelial weight and sclerotia production.

References

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How to cite this article

Satyadev Prajapati, Naresh Kumar, Shailesh Godika, Lalita Lakhran, Shivam Maurya and Sunil Kumar (2022). Assessment of Different Media on Growth and Sclerotia Formation of Sclerotinia sclerotiorum causing Sclerotinia Rot of Chilli under Laboratory conditi