INTRODUCTION Wheat (Triticum aestivum L.) is one of the major staple food crop feeding about 35 % of the world population. The global production of wheat is about 766 million tons and it is cultivated across nearly 216 million hectares in more than 125 countries (Sharma et al., 2021). The crop is self-pollinated and allohexaploid with chromosome number 2n =6x = 42 (Bonjean et al., 2001). Wheat is a rich source of complex carbohydrates (74-77%) and protein (11-15%) and poor source of sodium, amino acids and total fat (Dziki et al., 2021). The existing and projected high population growth rates would worsen the gap between wheat output and demand, adding to the country's limited food supplies. This situation emphasizes the importance of wheat breeding in increasing current productivity. Plant breeding is based on the notion of genetic variability. It could be developed by hybridization or mutations followed by selection (Addisu and Shumet 2015). Hybridization is tedious, time consuming, labour intensive and expensive nature of manual emasculation and pollination (Yahaya et al., 2020). Mutation breeding provides the advantage of being able to change specific qualities in otherwise acceptable varieties in a shorter amount of time by inserting some relevant variant. The Food and Agriculture Organization of the United Nations/International Atomic Energy Agency's Mutant Variety Database (FAO/IAEA-MVD data 2019) reports on 3,275 accessions from 225 species generated and freely disseminated by the FAO/IAEA. In crops, changes in the gene structure and sequence can be randomly caused by rupturing the DNA through physical and chemical mutagens (Viana et al., 2019). Nucleotide substitution causes random point mutation in plant material. The use of Sodium Azide and Hydroxyl Amine to induce mutants has been reported by number of workers (Weldemichael et al., 2021), (Ibukun et al., 2019) and (Kirtane et al., 2018). Keeping this in view, the present investigation was carried out to determine the effect of SA and HA on seed characteristics viz., germination percentage, shoot length, root length, seed vigour index-I, dry weight and seed vigour index-II. Different doses of HA and SA were also evaluated for vitality of the seeds. MATERIALS AND METHODS The study was conducted during Rabi 2021-2022 at Genetics and Plant Breeding Laboratory, School of Agriculture, ITM University, Gwalior (M.P.). Experimental material: “MP-3382” Matures in 119 days, high yielding (5975 kg/ ha), bold seeded, multiple resistances and heat tolerant. Recommended for irrigated, timely sown conditions of M.P. “RVW-4106”: It matures in 115-120 days. Resistant to black and brown rust disease. Average yield is 5035 kg/ha. Recommended for late sown irrigated conditions of M.P. Chemical Treatment: To begin with experiment, selected seeds were soaked in distilled water for 6 hours and the wet seeds were treated with different concentrations of HA and SA for six hours (Jeberson et al., 2020). The seeds were treated with 0.2%, 0.3%, 0.4%, 0.5% and 0.6% HA, whereas, 0.02%, 0.03%, 0.04%, 0.05% and 0.1% SA. The untreated seeds served as control. The treated seeds were washed thoroughly in running water for two hours to terminate the reaction of chemical mutagen and to leach out the residual chemicals. We have total 11 treatments including control for each variety. The treatment details are given in table 1. Observations to be recorded: For the assessment of seed quality parameters, 100 seeds of each treatments and replication including control were sown in petridish cover by blotting paper and half filled with water. Each of the treatments were replicated four times. The petridish were kept in seed germinator at 25±1ºC temperature and relative humidity was 85 ± 1 %. Observations were recorded for six characters i.e., germination percentage, root length, shoot length, seed vigour index – I, dry weight and seed vigour index – II. Statistical analysis: Data were collected and subjected to Analysis of Variance by using OP STAT (O.P. Sheoran Programmer, Computer Section, CCS HAU and Hisar) and significant mean was separated using t-test (One factor analysis). RESULTS AND DISCUSSION Analysis of Variance (ANOVA) revealed highly significant variation among the treatments for both the varieties for all six characters under study as shown in Table 2 and 3. The observation on six characters viz., germination percentage, root length, shoot length, seed vigor index -1, dry weight and seed vigor index –II on two different varieties; MP-3382 and RVW-4106 has been analyzed for the inference of result discussed as under. The effect of different doses of mutagens SA and HA is depicted through Fig 2. The graph represents the effect of the mutagens on two varieties with four characters. For the character Germination Percentage, maximum germination was found in MP-3382 for 0.4% HA (98%) which is more than control (95%). Least value of germination (83%) was found in RVW-4106 for 0.1% SA. The treatment V1T9 was observed to display maximum Root and Shoot length (11.31 cm and 11.20 cm) respectively. Least values of root and shoot length (3.26 cm and 2.85 cm) was observed in V2T6. Maximum Seed Vigor Index-I was again observed in same treatment V1T9 (2205.98) because the germination percentage as well as both root and shoot length was highest in this treatment as compared to control (1899.10) . The SVI of V1T9 was evaluated to be higher than 306 degrees. The character Dry Weight depends upon seedling length as more the seedling length more will be the weight therefore maximum dry weight was obtained in V1T9 because of maximum seedling length. The character SVI-II depends upon germination percentage and dry weight. Both the parameters displayed maximum magnitude for the treatment V1T9. Therefore, it is obvious to have maximum SVI-II (37.89) for the treatment T9. As per the Table 4, it is clearly evident that the different doses of mutagens created a significant variability for the characters still no such dose is validated which may act as LD50. All the treatments were found to be sub vital in survivability. In total, it was analyzed two categories of effect of mutagens. Increase in the magnitude and Decrease in the magnitude. An increase in the values of all the seed parameters taken under study can be explained due to increased embryonic growth coupled with high rate of mitotic cell division. Similar pattern of mutagenic effect has been recorded by Sharma et al., (2015), Irfaq and Nawab (2001); Jain et al. (2015). The mutagen Sodium Azide displayed delayed or inhibition in all the characters which might be due to suppressed enzymatic activities, hormonal imbalance and slow down of physiological process necessary for seed germination .This result is in synchronization with Herwibawa et al. (2018) in chill pepper, Abu et al., (2019); Ibukun et al. (2019); Akinyosoye (2020) in maize and Julia et al. (2022) in Indian Mustard.