Biogenic Synthesis of Zinc Nanoparticles using Wheat Root extract and endophytic Bacterium and their Effect on Wheat Germination and Seedling Vigour Index

Author: Patil Vishal N., Moharil M.P., Sakhare S.B., Ingale A.P., Bharad S.G., Hadole S.S., Gade A.K., Ghorade R.B. and Kharche V.K.

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Abstract

Nanoparticles are now used much more frequently in consumer goods as a result of the development of nanotechnology. This raises questions about how they may affect the health of plants. In present study, Zinc Oxide nanoparticles (ZnO NPs) were synthesized from wheat root extract and endophytic bacterium by green synthesis method. The synthesized nanoparticles were characterized by UV-visible spectrophotometry which showed an absorbance peak at 362 nm and 365 nm and particle size analysis showed that an average size of zinc oxide nanoparticles synthesized from wheat root extract was 19.66 nm and zinc oxide nanoparticles synthesized from endophytic bacterium was 37.3 nm, respectively. Different concentrations ZnO NPs (250, 500, 750 and 1000 ppm) were used to treat wheat seeds in order to assess their impact on seed germination. Significant effects on germination percentage, root length, shoot length, seedling length, root dry weight, shoot dry weight and seed vigour index (I and II) were seen at 500 ppm. Moreover, a decrease in all parameters that determined the negative effect of nanoparticles at higher concentrations was observed as ZnO NP concentration increased (to 750 ppm). Consequently, the findings of this study contributed that wheat root extract and endophytic bacterium may be employed as suitable sources for the green synthesis of ZnO NPs and that ZnO NP treatment had a substantial impact on seedling growth and Seed vigour index.

Keywords

Wheat root extract, Endophytic bacterium, ZnO NPs, SVI

Conclusion

Present study concluded, ZnO NPs can be synthesized by using wheat root extract and endophytic bacterium as a reducing agent. UV-visible spectroscopy and Particle size analysis indicated the synthesis of ZnO NPs. The significant increase was observed in germination percentage, root length, shoot length, seedling length, root dry weight, shoot dry weight, seed vigour index I and seed vigour index II at different concentrations of ZnO NPs treatment. The maximum increase in all parameters was exhibited at 500 ppm concentration. Further, increase in concentration (750 ppm) showed a reduction in all parameters that was possible due to the toxicity of nanoparticles at higher concentration.

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How to cite this article

Patil Vishal N., Moharil M.P., Sakhare S.B., Ingale A.P., Bharad S.G., Hadole S.S., Gade A.K., Ghorade R.B. and Kharche V.K. (2023). Biogenic Synthesis of Zinc Nanoparticles using Wheat Root extract and endophytic Bacterium and their Effect on Wheat Germination and Seedling Vigour Index. Biological Forum – An International Journal, 15(5): 128-135.