Objective: In this investigation, we have focused work on isolation, identification, biomass production, synthesis and evaluation of metabolites of economic importance from a native microalgal strain Haematococcus pluvialis KUDBT18. The study includes assessment of cultivation parameters, nutritional requirements for growth, observation of morphological and physiological changes with response to nutrient stress. Materials and methods: The isolate was identified by using microscopy and molecular techniques and cultivated photoautotrophically by using Bold Basal Media (BBM). Inoculum from seed culture 10% was added to 2000ml culture media. The physiological stress was induced by adding 0.1mM NaCl to 200ml media for synthesis of secondary metabolites with response to saline stress. Results and conclusion: The fresh water microalga isolated from Karnataka University Campus (KUC) is identified and named as Haematococcus pluvialis KUDBT18. The partial sequence 18S rRNA ribosomal gene was sub

Bold Basal Media, Carotenoids , Haematococcus pluvialis,, Photoautotrophic culture, Nutrient stress.

Molecular identification of Haematococcus pluvialis KUDBT18 strain confirmed by sequencing of 18S gene from 18S rRNA subunit. Partial sequence deposited to NCBI data repository with Gene bank accession number MH201223.1, hence the organism authenticated. The culture of Haematococcus grown on previously described growth media with addition of one or more combination of vitamins found to enhance the growth. Saline stress induction was responsible for encystment and production of secondary carotenoids. Haematococcus pluvialis is one of the industrially important organisms in trend it is reported from very few places in India as its existence is sporadic thus isolation, identification and maintenance of pure unialgal axenic culture is a challenging task. Hence in present study, experimental techniques developed for isolation and maintenance of pure cultures of KUDBT18 aiming its future use as promising neutraceutical compound. Techno economic assessment of the experimental design may hel

Shinge, M.R. and Shivasharana, C.T. (2019). Biomass Production and Extraction of Secondary Metabolites from a Fresh Water Microalga Haematococcus pluvialis KUDBT18 under Photoautotrophic Cultivation. Biological Forum - An International Journal, 11(2): 14