Changes in Poly Phenoloxidase and Peroxidase due to Bacterial Blight Disease in Clusterbean

Author: Anita Jat* and P.S. Shekhawat

Download PDF

Abstract

The activity of polyphenol oxidase and peroxidase enzyme was also disturbed in infected leaves of clusterbean. It was observed that amount of polyphenol oxidase and peroxidase activity was less in extracts prepared from healthy leaf tissues. The activity in infected leaves was more than healthy leaves. Therefore, the activity of polyphenol oxidase and peroxidase increased due to bacterial blight disease in clusterbean. Polyphenol oxidase per cent change in optical density/min/ml maximum was recorded in cultivar RGC-1055 (89.47 & 88.00) followed by RGC-1017 (85.00 & 80.76), RGC-986 (80.95 & 73.91), RGC-936 (45.45 & 71.87) and RGC-1031 (30.00 & 71.42) at 30 and 45 days old plants respectively. Peroxidase per cent change in optical density/min/ml maximum was recorded in cultivar RGC-1055 (52.63 & 51.56) followed by RGC-1017 (44.23 & 38.70), RGC-986 (34.88 & 30.18), RGC-936 (23.95 & 30.00) and minimum in RGC-1031 (9.34 & 17.43) 30 and 45 days old plants respectively.

Keywords

Clusterbean, Bacterial blight, Xanthomonas, Cultivar, Enzyme and Polyphenol oxidase.

Conclusion

As the disease progressed, the polyphenol oxidase activity increased in all the five cultivars. The activity in infected leaves was more than healthy leaves. The per cent change in activity was maximum in infected cultivar RGC-1055 (89.47 & 88.00) and minimum in RGC-1031 (30.0 & 71.42) at 30 and 45 days old plants respectively. The activity of peroxidase greatly increased in bacterial blight disease infected leaves of cultivar RGC-1055 (52.63% & 51.56%) and minimum in RGC-1031 (9.34% & 17.43%) at 30 and 45 days old plants respectively.

References

-

How to cite this article

Anita Jat and P.S. Shekhawat (2022). Changes in Poly Phenoloxidase and Peroxidase due to Bacterial Blight Disease in Clusterbean. Biological Forum – An International Journal, 14(1): 1017-1020.