In agriculture, Bacillus species are widely used to stimulate plant growth and to protect the plants from pathogens and other environmental stresses. In this present study, six Bacillus spp. were isolated from the rhizosphere soil of grapes and tomato and identified based on the morphological and biochemical features such as, Starch hydrolysis, Catalase test, Voges – Prokaeur test, Methyl red test, Gelatin hydrolysis test and KOH test and confirmed through Molecular characterization by using universal primers such as 27F and 1492R and got the amplicon size of 1500bp. Moreover, the confirmation of Bacillus spp. is done byusing 16S rRNA sequencing. This made to understand the nature of the bacteria present in the soil microbial community.

Bacillus species, Biochemical tests, Characterization, Universal 27F and 1492 R

Bacillus spp. perform well in the rhizosphere and phylloplane through different mechanisms viz., competition for nutrient uptake from the soil and induction of plant defense response against the plant diseases. However, the applicability of Bacillus spp. was limited due to their inconsistent efficiency in disease reduction under different field conditions. Thus, better formulation strategies have to be developed for the bacterial biocontrol agent, Bacillus spp. to make them more durable with improved biocontrol efficacy.

Veerathilagam D., Kannan R., Parthiban V.K., Vincent S. and Bhuvaneswari K. (2022). Characterization of Bacillus spp. Isolated from different Rhizosphere Soils. Biological Forum – An International Journal, 14(4a): 29-32.