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Characterization of Twenty One Isolates of Beauveria bassiana and Study their Bio-efficacy against Nodostoma subcostatum

Author: B. Temjenmenla, K.C. Puzari, A. Bhattacharyya, P.D. Nath and Pranab Dutta*

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Abstract

Considering the negative effects of chemical pesticides have led to emphasis on use of biological control of scarring beetle. B. bassiana, the white muscardine fungus was reported to be effective against banana beetle (N. subcostatum) in Assam, India. Information on the potentialities of B. bassiana for the management of N. subcostatum is scanty. Twenty one (21) isolates of B. bassiana were made banana leaf and fruit scarring beetle during the survey conducted in four North East States viz., Assam, Arunachal, Mizoram and Nagaland and all the isolates were characterized. Out of the different tested concentrations of B. bassiana evaluated in laboratory against N. subcostatum, concentration of 1 × 107conidia/ml of water was found effective with highest mortality of 89.17 per cent at 20 days post inoculation. All the 21 isolates of B. bassiana tested at 1 × 107 conidial concentration was found to be best with highest pathogenic to adult leaf and fruit scarring beetle causing mortalitie

Keywords

Beuveria bassiana, Nodostoma subcostatum, isolate, phylogenetic, genetic variability.

Conclusion

The present study showed that isolates of B. bassiana are highly pathogenic to N. subcostatum and studies on molecular characterization of the isolates proved the variability at genetic level among the isolates of B. bassiana of North East States. These isolates can be use as a potential bioagent for the management of the pest in organic cultivation of banana, thereby it will reduce the use of chemical pesticides and save the environment.

References

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How to cite this article

B. Temjenmenla, K.C. Puzari, A. Bhattacharyya, P.D. Nath and Pranab Dutta (2022). Characterization of Twenty One Isolates of Beauveria bassiana and Study their Bio-efficacy Against Nodostoma subcostatum. Biological Forum – An International Journal, 1