Indexing

Cloning of rbcL region from Cicer arietinum L. for species specific plastidial vector

Author: Somayeh Kalili Kolikand and Bahram Baghban Kohnehrouz

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Abstract

Chickpea is a good source of protein and carbohydrate among crop plants. There are some agronomic and economically important traits could be improved by transgenic and transplastomic research in this field crop. Despite the high yield potential of chickpea (over 4000 Kg.ha-1) transgenic breeding is going to develop new cultivars against biotic and abiotic stresses as well as modern industrial usages. To do this by transplastomic approach chickpea specific plastidial vector is an essential need which is pursued in this work. The nucleotide sequence of chickpea organellar genome of plastid was used for determining the rbcL regional to design specific primer set using on line Primer blast software. Total genomic DNA was extracted from seedling leaves and used for PCR reaction as template. Expected length of amplified rbcL fragment was confirmed by agarose gel electrophoresis. The fragment extracted from agarose gel (0.8 %) using the gel purification and extraction kits and used for the T

Keywords

chickpea, Cicer arietinum L, cloning, plastidial vector, rbcL

Conclusion

Chickpea is a good source of protein and carbohydrate among crop plants. There are some agronomic and economically important traits could be improved by transgenic and transplastomic research in this field crop. Despite the high yield potential of chickpea (over 4000 Kg.ha-1) transgenic breeding is going to develop new cultivars against biotic and abiotic stresses as well as modern industrial usages. To do this by transplastomic approach chickpea specific plastidial vector is an essential need which is pursued in this work. The nucleotide sequence of chickpea organellar genome of plastid was used for determining the rbcL regional to design specific primer set using on line Primer blast software. Total genomic DNA was extracted from seedling leaves and used for PCR reaction as template. Expected length of amplified rbcL fragment was confirmed by agarose gel electrophoresis. The fragment extracted from agarose gel (0.8 %) using the gel purification and extraction kits and used for the T

References

Chickpea (Cicer arietinum L.) is one of the oldest and most widely consumed legumes in the world due to relatively high protein content and wide adaptability as a food grain. It is the second most widely grown legume in the world (FAO, 2008). Its protein quality is better than other legumes such as pigeon pea, black gram and green gram. Chickpea has significant amounts of all the essential amino acids except sulfur containing types, which can be complemented by adding cereals to daily diet. Chickpea has several potential health benefits and in combination with other pulses and cereals, it could have beneficial effects on some of the important human diseases like cardiovascular, type 2 diabetes, digestive diseases and some cancers. Overall, chickpea is an important pulse crop with a diverse array of potential nutritional and health benefits (Kaur and Singh, 2005). There is a growing demand for chickpea due to its nutritional value. In the semi-arid tropics chickpea is an important

How to cite this article

Somayeh Kalili Kolikand and Bahram Baghban Kohnehrouz (2015). Cloning of rbcL region from Cicer arietinum L. for species specific plastidial vector .Biological Forum – An International Journal 7(1): 1599-1604.