Comparative Study of Different Diagnostic Modalities for the Detection of Malaria Parasite at a Tertiary Care Centre Kanpur

Author: Deepak Sameer, R. Sujatha, Shrawan Kumar and Nashra A.

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Abstract

Globally, the incidence of malaria continues to increase and India contributes to one third of cases of malaria globally with the highest incidence reported by Plasmodium vivax species. Comparative study of different diagnostic modalities for the detection of malaria parasite at a tertiary care centre Kanpur. The study was carried out in the Department of Microbiology and Central Research Lab of RMCH & RC, Kanpur, for a period of 1 year March 2021 to March 2022. A total of 151 whole blood samples were collected & examined by Peripheral blood smear (PBS) stained with Giemsa stain, rapid diagnostic test (RDT) for detection of antigen and CBS (Centrifuged buffy coat smear) tests respectively. The Ethical Clearance was taken from the Ethical Committee of the institution. Out of the total 151 Malaria suspected cases, 10 positive cases of Plasmodium vivax species were detected by RDT, CBS and 6 by PBS tests. 7 (70%) were male and 3 (30%) were female, the maximum number of cases were in 41-50 years followed by 1-30 years. The comparative performance of RDT, CBC and PBS were analysed, the RDT and CBS showed a sensitivity of (80%), specificity (98.58%) PPV (80%), NPV (98.58%) and accuracy (97.35%), the sensitivity of PBS is 60%, specificity 98.58%, PPV(70%), NPV(98.58%) and accuracy (96.02%) respectively. In our study the sensitivity, specificity of PBS is lesser than RDT and CBS, hence owing to the high number of false negatives in microscopy, it is necessary to reinforce training in microscopy, the “Gold Standard” in endemic areas, especially for confirmation of clinical diagnosis.

Keywords

RDT, CBS, sensitivity and specificity

Conclusion

In our study the sensitivity, specificity of PBS is lesser than RDT and CBS, hence owing to the high number of false negatives in microscopy, it is necessary to reinforce training in microscopy, the “Gold Standard” in endemic areas, especially for confirmation of clinical diagnosis.

References

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How to cite this article

Deepak Sameer, R. Sujatha, Shrawan Kumar and Nashra A. (2023). Comparative Study of Different Diagnostic Modalities for the Detection of Malaria Parasite at a Tertiary Care Centre Kanpur. Biological Forum – An International Journal, 15(5): 165-168.