Author: Roja S., Sunil Kulkarni*, Gururaj Sunkad, Ajithkumar K. and Shobharani M.
Fungi are heterotrophic organisms that rely on external sources of sustenance for their growth, reproduction, and survival. It is critical to provide all of the necessary nutrients in the form of media in order to grow fungus in a laboratory setting. Temperature and relative humidity are crucial external factors to consider. Temperature has an impact on practically every function of fungi, including growth and sporulation, and relative humidity is crucial in the progression of the disease into epidemic form. At the Department of Plant Pathology, UAS Raichur, an experiment was done to investigate the effects of different medium, temperature, and relative humidity on the growth of Colletotrichum truncatum, which causes soybean pod blight. Solid media PDA was found to be best for good growth subsequently oat meal agar with excellent sporulation. Among liquid media tested, Richard’s broth was found significantly superior over other broths with dry mycelial weight of 427.67 mg, followed b
Soybean, pod blight, temperature, relative humidity, solid media, liquid media.
According to the results of this study, potato dextrose agar and Richard's broth are the best solid and liquid media for cultivating fungus, respectively. Furthermore, a temperature of 25 °C and a relative humidity of 80% were optimal conditions for the pathogen growth.
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Roja, S.; Kulkarni, S.; Sunkad, G.; Ajithkumar, K. and Shobharani, M. (2022). Cultural and Physiological Requirements of Colletotrichum truncatum causing Soybean Pod Blight. Biological Forum – An International Journal, 14(1): 85-91.