Decoding Pratylenchus thornei: Reproductive Insights in Carrot Disc Culture and Bioagent Efficacy for Precision Chickpea Nematode Management

Author: Vedant Gautam, Ashish Kumar, R Shivram Krishnan, Stuti Sharma, Radheshyam Sharma, Vibhootee Garg, Sonu Sharma, Sanjay Kharte, Rajkumar Bajya and Ravi Nagar

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Abstract

This research delves into the reproductive behavior of Pratylenchus thornei in carrot disc culture, explores its vertical distribution in soil, and evaluates the effectiveness of various bioagents in mitigating nematode impact in chickpea cultivation. In carrot disc culture, the study reveals a maximum reproductive fitness of 19.56 fold after 40 days of inoculation, accompanied by a final nematode population of 489, comprising 84 females, 244 juveniles, and 161 eggs. Contrastingly, a minimum nematode population of 94 was observed after 20 days, with a reproductive fitness of 3.76. Extending the incubation period from 35 to 40 days yielded the highest reproductive rate, a pattern consistent with studies on P. neglectus and P. zeae in carrot disc cultures. Evaluation of bioagents for nematode management demonstrated their overall effectiveness, with Purpureocillium lilacinum exhibiting maximum efficacy. Utilizing this bioagent as seed treatment in JG 62 chickpea variety resulted in a final nematode population of 18.65 per 500 cc soil, 7.13 nematodes per gram of root, and a maximum plant height of 11.3. Similar efficacy was reported for T. harzianum and P. lilacinum in other studies, potentially attributed to the activation of defense genes. In conclusion, this study not only provides insights into the reproductive dynamics and vertical distribution of P. thornei but also underscores the promising potential of bioagents, particularly Purpureocillium lilacinum, for effective nematode management in chickpea cultivation.

Keywords

Pratylenchus thornei, Carrot disc culture, Bioagents, Nematode management, Purpureocillium lilacinum

Conclusion

In this study, the reproductive dynamics of Pratylenchus thornei in carrot disc culture, its vertical distribution in soil, and the efficacy of bioagents in chickpea nematode management were thoroughly investigated. Carrot disc culture experiments revealed significant variations in reproductive fitness over different incubation periods, with a maximum fold increase of 19.56 observed after 40 days. The vertical distribution of P. thornei in soil showed a peak population at 12-16 cm depth, emphasizing the impact of chickpea cultivation practices on nematode presence. Evaluation of bioagents demonstrated their effectiveness, with Purpureocillium lilacinum exhibiting maximum efficacy in reducing nematode populations in soil and roots.

References

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How to cite this article

Vedant Gautam, Ashish Kumar, R Shivram Krishnan, Stuti Sharma, Radheshyam Sharma, Vibhootee Garg, Sonu Sharma, Sanjay Kharte, Rajkumar Bajya and Ravi Nagar (2023). Decoding Pratylenchus thornei: Reproductive Insights in Carrot Disc Culture and Bioagent Efficacy for Precision Chickpea Nematode Management. Biological Forum – An International Journal, 15(10): 995-1000.