Degradation of Keratin by Keratinase Enzyme Producing Bacillus zhangzhouensis using Insilico Protocols

Author: Rajee T. and Selvamaleeswaran P.

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Abstract

The management of keratinous waste generated from different industries is becoming a major challenge across the world, especially in poultry farming. Therefore, there is a need for modern and eco-friendly approaches to disposing of waste products. Due to the environmental challenges of poultry farming, it is necessary to isolate microbial keratinase enzymes with high catalytic functions and identify its mechanisms. Therefore, in the present study, our goal was to identify the potential of keratinase producing isolates that degrade feathers containing keratin through docking studies. The protein structure was predicted using the 16srRNA gene sequence of Bacillus zhangzhouensis, and the keratin structure was also predicted with the Insilico method. Then, protein-protein docking studies are performed using an automated protein docking server and their 3D molecular binding interactions are discussed in detail. The overall results clearly show that the amino acids of the enzyme, keratinase efficiently bind at the active sites (motifs) of keratin. Despite these challenges, keratinase enzymes have been successfully used to reduce the levels of keratin in soil, making them a viable option for remediating the environment.

Keywords

16sr RNA sequence, Bacillus zhangzhouensis, Keratin, Protein –Protein Docking

Conclusion

Keratin is abundantly found in nature, and the volume of keratin-rich waste keeps increasing each year. Keratinase plays an important role in the recycling of keratin and is hence used in different fields. Our protein-protein docking studies revealed that the keratinase enzyme of Bacillus zhangzhouensis binds well with the feather keratin protein of chicken and aids in the breakdown of disulfide bonds present in keratin. These Insilico results provide evidence that the enzyme of the bacterial sps is eligible for the degradation of keratin.

References

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How to cite this article

Rajee T. and Selvamaleeswaran P. (2023). Degradation of Keratin by Keratinase Enzyme Producing Bacillus zhangzhouensis using Insilico Protocols. Biological Forum – An International Journal, 15(5): 07-16.