Indexing

Detection of Squalene compound in Ganoderma lucidum through thin-layer Chromatography (TLC)

Author: B. Sangeetha, A.S. Krishnamoorthy, P. Renukadevi, V.G. Malathi and D. Jeya Sundra Sharmila

PDF Download PDF

Abstract

Thin-layer chromatography is a simple, inexpensive, easy, and reliable method widely used for detection of various toxins present in food and plant materials in Plant Pathology. Thin layer chromatography can also be used to identify the nature of different plant compounds: anti-oxidative, antibacterial, or antifungal. In this study, we have used this method to detect the antiviral compounds in the Ganoderma lucidum. Ganoderma lucidum, one of the medicinal mushrooms, has antiviral properties against the groundnut bud necrosis virus infecting tomatoes. The antiviral compound squalene has already been reported. Hence, the presence of squalene in different extracts of G. lucidum, viz., secondary metabolites, mycelial extracts, and basidiocarp extract, was analysed by the thin layer chromatography (TLC) method. The results of red brick colour formation and refractive index in the different extracts of G. lucidum have confirmed the presence of squalene. Analysing the costs of the various techniques, TLC emerged as the most affordable method for sample analysis. TLC is the most convenient and economical method for finding compounds in various microorganisms.

Keywords

Ganoderma lucidum, Squalene, Thin-layer chromatography (TLC)

Conclusion

Various chromatographic methods were used for the analysis of secondary metabolites and compounds from the samples. Apart from all, thin-layer chromatography is an easy and cost-effective, and conventional method widely used to find the aflatoxins and various toxins from food products. The presence of an antiviral compound viz., squalene from different extracts of G. lucidum was easily confirmed by this TLC method.

References

-

How to cite this article

B. Sangeetha, A.S. Krishnamoorthy, P. Renukadevi, V.G. Malathi and D. Jeya Sundra Sharmila (2023). Detection of Squalene compound in Ganoderma lucidum through thin-layer Chromatography (TLC). Biological Forum – An International Journal, 15(8a): 257-261.