Development of 16S rRNA Polymerase Chain Reaction and its Comparison with BCSP31 Polymerase Chain Reaction for Identification of Brucella

Author: S. Rajagunalan, Soni Doimari, S. Murugavel and D.K. Singh

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Abstract

The present study was carried out to develop a 16S rRNA-based polymerase chain reaction (PCR) assay for the identification of Brucella isolates at the genus level and evaluate its efficiency by comparing it against the BCSP31 PCR assay. Oligonucleotide primers specific for the 16S rRNA gene of Brucella were designed and compared with published primers specific for BCSP31 for the identification of Brucella at the genus level. A total of 46 Brucella isolates (11 standard isolates and 35 Brucella melitensis clinical isolates) were used for amplification with both the primers. The sensitivity and specificity of the primer were also evaluated. Both the PCR assays demonstrated specificity in the identification of Brucella, but the 16S rRNA PCR assay exhibited lower sensitivity compared to the BCSP31 gene-specific PCR assay. The 16S rRNA PCR assay is suitable for genus-level identification of Brucella isolates, although further validation is needed for its application in direct clinical sample detection.

Keywords

Brucella, Diagnosis, PCR, 16S rRNA, BCSP31

Conclusion

This study developed a PCR assay targeting the 16S rRNA gene and evaluated its utility in screening Brucella isolates, demonstrating high specificity but lower sensitivity compared to BCSP31-targeted assays. Despite their lower sensitivity, these primers remain valuable for screening suspected Brucella isolates.

References

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How to cite this article

S. Rajagunalan, Soni Doimari, S. Murugavel and D.K. Singh (2023). Development of 16S rRNA Polymerase Chain Reaction and its Comparison with BCSP31 Polymerase Chain Reaction for Identification of Brucella. Biological Forum – An International Journal, 15(10): 1714-1718.