Dissipation analysis of Difenaconazole (25 EC) on apple under temperate conditions of Kashmir

Author: Sakib N., Rather M.F., Bhat Z.A., Mukhtar M., Wani A.A., Sharma M.K. , Sultan A., Bhat M.A.

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Abstract

In present study, the dissipation pattern and persistence of foliar application of difenaconazole (25 EC) on leaves and fruits of apple were investigated at 300 g ai ha-1 and 600 g ai ha-1. The samples were obtained at different time intervals viz., 0, 1, 3, 5, 7, 10, 15, 20, 25, and 30 days after treatment. The cost-effective, efficient, and robust QuEChERS method coupled with a gas chromatography mass spectrometery was used to analyze the residual behaviour of difenaconazole. The residual concentration in leaves and fruits dropped below the detection limit on 30th day at 300 g ai ha-1 and 25th and 30th day at 600 g ai ha-1 during 2021. The mean recovery rates in leaves and fruits ranged from 84.80 to 101.6 per cent and the relative standard deviation was 1.69 to 3.97 per cent. The half-life of difenaconazole in leaves and fruits were 9.9 to 10.19 days and 5.21 to7.3 days at single and double dose, respectively. In terms of pre-harvest intervals (PHI), the time required for residues to decrease to permissible levels (maximum residue limits) ranged from 6.8 to 11.75 days for the single dose and from 3.65 to 8.54 days for the double dose.

Keywords

Difenaconazole, Dissipation, Residue, Half-life, QuEChERS, Recovery

Conclusion

From the present investigation it was clearly depicted that the dissipation behaviour of difenaconazole showed the different dissipation rates on leaves and fruits of apple. In the context of leaf samples, the observed half-life and the corresponding waiting period were 9.9 and 10.19 days at the recommended dosage, and 6.8 and 11.75 days at double the recommended dosage. As for fruit samples, the half-life and associated waiting period were 5.21 and 7.53 days at the recommended dose, and 3.65 and 8.54 days at double the recommended dose. It's worth noting that the reduction of residues in both dosages adhered to the principles of first-order kinetics for degradation.

References

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How to cite this article

Sakib N., Rather M.F., Bhat Z.A., Mukhtar M., Wani A.A., Sharma M.K. , Sultan A., Bhat M.A. (2023). Dissipation analysis of Difenaconazole (25 EC) on apple under temperate conditions of Kashmir. Biological Forum – An International Journal, 15(10): 498-501.