Effect of 6-Benzylaminopurine (BAP) and Kinetin (Kn) on Callus Induction under In vitro Culture of Aloe vera

Author: Sarfraz Ahmad*, M.L. Jakhar, D.K. Gothwal, Manohar Ram, B.L. Kumhar and G.L. Kumawat

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Abstract

The BAP (6-benzylaminopurine) and Kn (Kinetin) are important cytokinins, commonly utilizes in plant tissue culture for micropropagation of various plant species. However, their regeneration response under in vitro condition highly dependent on the culture condition especially concentration of growth regulators. In the present investigation, effects of different levels of BAP and Kn (0.5 to 6.0 mg/l) were evaluated for callus induction in lateral shoot explant of Aloe vera. Differential concentrations of these growth regulators had shown variable effects on callus proliferation in MS (Murashige and Skoog) medium. In BAP incorporated medium, maximum callus induction (0.92 g) was obtained at the concentration of 5.0 mg/l BAP, which was characterized with yellow colour and semi compact texture. However, highest callus weight induced in Kn fortified medium was 1.25 g obtained at 4.0 mg/l. Induced callus morphologically characterizes with whitish yellow colour and friable textured. Among the

Keywords

Aloe, BAP, Callus, Cytokinin, Kinetin, Micropropagation.

Conclusion

In the present investigation, effects of different levels of BAP and Kn were evaluated for callus induction in lateral shoot explant of Aloe vera. In BAP incorporated medium, maximum callus induction (0.92 g) was obtained at the concentration of 5.0 mg/l, while highest callus weight induced in Kn fortified medium was 1.25 g at 4.0 mg/l. Consequently, Kn found more proficient than BAP for callus proliferation in lateral shoot explant. The results of current investigation give an idea of effectiveness of BAP and Kn for morphogenetic effects on callus proliferation. Thus, for best economical use, the current study may help in the identification of suitable growth regulator with appropriate level for getting maximum callus induction response for in-vitro micropropagation of Aloe vera.

References

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How to cite this article

Sarfraz Ahmad, M.L. Jakhar, D.K. Gothwal, Manohar Ram, B.L. Kumhar and G.L. Kumawat (2022). Effect of 6-Benzylaminopurine (BAP) and Kinetin (Kn) on Callus Induction Under In vitro Culture of Aloe vera. Biological Forum – An International Journal, 14(1)