Sclerotinia stem rot caused by Sclerotinia sclerotiorum in rapeseed-mustard is a soil borne, necrotrophic pathogen. In this study the effect of Carbon and nitrogen sources on growth and sclerotial development of Sclerotinia sclerotiorum. Among the nitrogen sources, the maximum mycelium growth was recorded in ammonium sulphate @2% at 3 and 5 DAI. The maximum number and size of sclerotia were obtained in ammonium nitrate @2%. Among the carbon sources, the maximum mycelium growth was recorded in sucrose @2%. The maximum number of sclerotia per plate was recorded in sucrose @2%. The maximum size of sclerotia was recorded in D-glucose @2%. The present study helped in determining the carbon and nitrogen sources favourable for growth and sclerotial development of the pathogen.

Sclerotinia sclerotiorum, Carbon, Nitrogen, Ammonium sulphate, Sucrose

Among the nitrogen sources, the maximum mycelium growth was recorded in ammonium sulphate@2% at 3 and 5 DAI. The maximum number and size of sclerotia were obtained in ammonium nitrate @2%. Among the carbon sources, the maximum mycelium growth was recorded in sucrose@2%. The maximum number of sclerotia per plate was recorded in sucrose@2%. The maximum size of sclerotia was recorded in D-glucose@2%. Thus, from the present study the nutritional requirement of Sclerotinia sclerotiorum affecting mustard crop was known.

Sonu Sharma, R.K. Pandya, Rajni Singh Sasode, Vijay Kumar Kashyap and Shivram Chandel (2023). Effect of Carbon and Nitrogen Sources on Growth and Sclerotial Development of Sclerotinia sclerotiorum. Biological Forum – An International Journal, 15(3): 543-546.