Pea (Pisum sativum L.) is one of the important pulse crop in India, having chromosome number is 2n=24 and family is Leguminaceae. Peas are a valuable vegetable for vegetarians and fruits and seeds, both green and mature, are rich in starch, proteins. Mutation is the ultimate source creating variation and Breeder always want wider variability among population. Mutation breeding can improve the genotype. For improvement in the population variability is required, which can be created by various methods like hybridization, soma clonal variation, polyploidy. Mutation is also play an important role in creating variability. The present study was carried out to determine effect of chemically induced mutation on yield contributing and seed quality attribute in Pea (Pisum sativum L.) in M1 generation. Seed were pre-soaked in distilled water for 6hrs and later treated with different concentration w/v (0.1, 0.15, 0.20, 0.25) of Sodium Azide and Hydroxyl Amine respectively for 6hrs. The experiment was planted Randomized block design. Observation in M2 generation showed significant variation on the germination, root and shoot length, vigour index I and II, seedling dry weight, mortality, survival, primary secondary branches, days to first flowering, length of pod, number of pods per plant, seed test weight. Most of the parameters decreased with increase in concentration of Sodium Azide. The result of study showed that Sodium Azide 0.25 and 0.20 are more effective to induce variability in population. A significantly was observed effects on plant height, germination, days to first flowering and Days to first flowering. Sodium Azide is more effective as compared to the Hydroxyl Amine in inducing genetic variability.

Pea, Mutant, Sodium Azide, Hydroxyl Amine, S10, Variability

The variation in the morphological features in the M1 mutant, S10 is an indication that Sodium Azide is more effective as compared to the hydroxyl amine in inducing genetic variability in the quantitative trades generally, differences in concentration of the mutagen significantly affected most of the parameters evaluated in the given varieties. The result of study showed that Sodium Azide significant effect on plant height, germination and days to first flowering and Number of pod per plant. Therefore, induce mutation is highly effective in creating variability of desirable trade in Pea for further reading program.

Sneha Vinod Bansod, Jai Prakash Gupta, Binod Kumar Pandeyand Sachin Rajesh Awachar (2022). Effect of Chemically induced Mutation on Yield Contributing and Seed quality Attribute in Pea (Pisum sativum L.). Biological Forum – An International Journal, 14(3): 1497-1500.