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Effect of Different Carbon and Nitrogen Sources on Mycelial Growth and Sclerotial Formation of Sclerotium rolfsii Sacc. causing Stem Rot of Wheat

Author: Vikram*, Praful Kumar and Ashwarya L. Tandon

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Abstract

Nutritional requirements in semi-synthetic media is very essential for growth and survival of S. rolfsii. However, there is little information on the nutritional requirements of S. rolfsii. Therefore, In vitro experiments were conducted at the Department of Plant Pathology, College of Agriculture (Indira Gandhi Krishi Vishwavidyalaya) Raipur, Chhattisgarh, to evaluate the effect of different carbon and nitrogen sources on mycelial growth and sclerotial formation of Sclerotium rolfsii Sacc. causing stem rot of wheat. The treatments comprised of the six-carbon sources viz., Glucose, Lactose, Maltose, Mannitol, Starch, and Sucrose, and six nitrogen sources viz., Glycine, Barium nitrate, Magnesium nitrate, Potassium nitrite, Sodium nitrate, and Urea with separate control, for both carbon and nitrogen, were maintained by using Dextrose as carbon source and Sodium nitrate as nitrogen source. Radial growth, number of sclerotia, mycelial density, colony shape, mycelia texture, sclerotia color,

Keywords

Sclerotium rolfsii, Stem rot, Wheat, Carbon source, Nitrogen source.

Conclusion

In this investigation, considerable variations in mycelial growth, and sclerotial development were observed using different N and C nutritional sources for S. rolfsii culture under laboratory conditions. However, to provide a greater understanding of the biology of S. rolfsii, further investigations into the effect on pathogen development under field conditions are needed. Such investigations would improve our understanding of the pathogen’s population dynamics in soil and help to implement effective disease control methods.

References

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How to cite this article

Vikram, Praful Kumar and Ashwarya L. Tandon (2022). Effect of different Carbon and Nitrogen sources on Mycelial Growth and Sclerotial Formation of Sclerotium rolfsii Sacc. Causing Stem Rot of Wheat. Biological Forum – An International Journal, 14(1): 14