Effect of different Solid Media and Temperature level on Growth of Cercospora canescens causing Cercospora Leaf Spot Disease in Mungbean

Author: Naresh Kumar*, Lalita Lakhran and Mahabeer Singh

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Abstract

The present paper describes the effects of various solid media and temperatures for the growth on Cercospora canescens. Efficacy of various solid media at the growth of Cercospora canescens were tested and resulted that in Malt Extract Agar medium (64.70 mm) was found with maximum mycelial growth of the tested pathogen observed by Potato Dextrose Agar (43.50 mm). The minimum mycelial growth was noticed in Corn Meal Agar (16.00 mm). The temperature studies revealed that highest mycelial growth (85.00 mm) became found at 25°C and minimum mycelial (20.33 mm) growth was found at 35°C However, 20°C, 30°C and 15°C also not favoured good growth of Cercospora canescens but differ significantly from growth at 25°C. Not growth was found at 40°C. It can be concluded that 25°C is the optimum temperature for mycelial growth of Cercospora canescens. Temperature plays important role among the external factors which influence the growth and reproduction of fungi. All the fungi have minimum tem

Keywords

Mungbean, Solid Media, Temperature, Cercospora canescens.

Conclusion

In the present study, the Malt Extract Agar medium was found with maximum mycelial growth of the tested pathogen followed by Potato Dextrose Agar medium. The minimum mycelial growth was noticed in Corn Meal Agar. The growth characters varied from concave to convex, raised, pluffy, whitish to light grey colony colour and regular to irregular colonies were noticed. Sporulation of the pathogen was also showed variation on different media. Good sporulation was recorded in Malt Extract Agar media and moderate sporulation was noticed in Potato Dextrose Agar. Poor sporulation was observed in Czapek’s Dox Agar media and no sporulation were observed in Richard’s Agar, Corn Meal Agar and Oat Meal Agar media. The temperature studies revealed that highest mycelial growth was observed at 25°C and lowest mycelial growth was observed at 35oC However, 20°C, 30°C and 15°C also not favoured good growth of Cercospora canescens but differ significantly from growth at 25°C. No growth was found a

References

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How to cite this article

Naresh Kumar, Lalita Lakhran and Mahabeer Singh (2022). Effect of different Solid Media and Temperature level on Growth of Cercospora canescens causing Cercospora Leaf Spot Disease in Mungbean. Biological Forum – An International Journal, 14(1): 694-698