Effect of Indole-3-acetic acid (IAA) on in-vitro Regeneration of Medicinal Plant-Gwarpatha (Aloe vera)

Author: Sarfraz Ahmad*, M.L. Jakhar, S.S. Rajput, Varsha Kumari, S.S. Punia and Madhu Yadav

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Abstract

The discovery and documentation of the role of plant growth regulators in tissue culture served as major thrust for the crop improvement. For getting maximum response, utilization of suitable growth regulator at appropriate concentration needs to be carefully balanced and controlled. Therefore, the present investigation was carried out to optimize the levels of Indole-3-acetic acid (IAA) for micropropagation of Gwarpatha [Aloe vera (L.) Burm. f.]. Lateral shoot explant was used for inoculation with incorporation of different concentrations of IAA (0.5 – 4.0 mg/L) in Murashige and Skoog (MS) medium. Diverse regeneration responses were observed for shoot multiplication, root induction and for callus induction at varied levels of IAA. Highest number of shoot per explant (2.90) was recorded in the treatment of 3.0 mg/L with 70 % induction frequency while, maximum number of root (4.10) was induced at 2.0 mg/L. Moreover, highest callus weight (1.24 g) was reported at 4.0 mg/L with yellow b

Keywords

Aloe vera, Callus, Gwarpatha, Indole-3-acetic acid, Micropropagation, IAA, Root induction, Shoot proliferation.

Conclusion

Indole-3-acetic acid (IAA) plays multidimensional role on regeneration of Aloe vera under in vitro condition. However, different concentrations showed variable responses for shoot multiplication, root induction and callus proliferation in lateral shoot explant. The best performing levels for shoot multiplication and root induction were obtained at 3.0 mg/L and 2.0 mg/L, respectively. Moreover, maximum callus was obtained at the concentration of 4.0 mg/L. These identified treatment levels of IAA further can be utilized for better economic use in the propagation of Aloe vera under in vitro condition.

References

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How to cite this article

Sarfraz Ahmad, M.L. Jakhar, S.S. Rajput, Varsha Kumari, S.S. Punia and Madhu Yadav (2022). Effect of Indole-3-acetic acid (IAA) on in-vitro Regeneration of Medicinal Plant-Gwarpatha (Aloe vera). Biological Forum – An International Journal, 14(1): 460-46