Effect of Media for in vitro Seed Germination of Vanda coerulea

Author: Himal Pokhrel* and Amitava Paul

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Abstract

Vanda Coerulea is a monopodial orchid and its propagation in nature through seed germination is difficult. So, in vitro propagation through germination of the seed using different media is being carried out for the study. For in vitro seed germination of Vanda coerulea, four basal media (MS, Mitra, Knudson C and Vacin & Went) were taken for the study. Four replications in each media were taken with eight culture bottles for each replication. 9-10 months old immature hand pollinated seed pods were used as a seed source. Standard media preparation procedure was followed and pH of the media was adjusted to 5.3. Sterilized seed pod was brought inside the laminar air flow for seed culture. After successful culture of seeds, the cultured bottles were kept at the culture room with outmost care under supervision. Color change and swelling of seeds were observed after few weeks of culture. Gradually swelling portion turned into Plbs in due course of time and emergence of shoots and roots was ob

Keywords

in vitro, seed pod, basal media, germination, Vanda coerulea.

Conclusion

The findings of the present study conclude that MS basal media can be used for in vitro propagation of Vanda coerulea as it can give maximum seed germination and shoot initiation in a short period of time.

References

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How to cite this article

Himal Pokhrel and Amitava Paul (2022). Effect of Media for in vitro Seed Germination of Vanda coerulea. Biological Forum – An International Journal, 14(1): 180-183.