Peroxidase, an antioxidant enzyme, is important in eliminating excess reactive oxygen species from earthworm cells. Insecticides such as neonicotinoid acetamiprid and imidacloprid are becoming more popular by the day in order to increase crop yields. The major goal of this study is to see how different doses of imidacloprid and acetamiprid affect the antioxidant enzyme peroxidase activity in earthworms Eisenia fetida and maintaining the right environment for properly measuring the enzymatic activities estimation was extremely challenging because the enzymatic activity of earthworms varied rapidly. During the current experiment, three dosages of acetamiprid (0.145 µg, 0.165 µg, and 0.188 µg) and imidacloprid (0.134 µl, 0.195 µl, and 0.280 µl) were tested on direct exposure in vials with a diameter of 3mm and 8 cm length. After 48 hours of exposure to acetamiprid, peroxidase activity was 0.775 and 0.858Umg-1 protein at concentrations of 0.165 µg and 0.188 µg respectively, and 0.8

Acetamiprid, imidacloprid, neonicotinoid, insecticides, Eisenia fetida, POD

Pesticides have a greater impact on Eisenia fetida, and the study found that POD activities are completely reliant on pesticide concentrations and direct exposure time. The activities of POD enzyme after 24hr and 48hr exposure rose as acetamiprid and imidacloprid concentrations increased, indicating the need to limit pesticide use to protect soil invertebrate flora.

Gill, P., Singh, D. Sharma, B.L., Gupta, R.K., Dahiya, T., Rao, D.S., Lather, D. and Kumar, N. (2021). Effect of Neonicotinoid Acetamiprid and Imidacloprid Insecticides on Antioxidant Peroxidase Activity in Earthworm Eisenia fetida. Biological Forum