This study investigates the influence of parity on milk yield and key compositional traits (fat%, protein%, and lactose%) in Murrah buffaloes. Milk samples were collected from clinically healthy animals across five parity groups (1st to 5th). The average morning milk yield increased from 4.2 ± 0.31 liters in parity 1 to a peak of 5.4 ± 0.45 liters in parity 4, before declining to 4.0 ± 0.34 liters in parity 5. Evening milk yield followed a similar trend, peaking at 5.1 ± 0.46 liters in parity 4. Fat percentage was highest in parity 3 (9.0 ± 0.61%), followed by parity 1 (8.6 ± 0.73%) and parity 5 (8.4 ± 0.68%), with the lowest value observed in parity 2 (7.5 ± 0.66%). Protein content showed a slight decreasing trend from 3.7 ± 0.10% in parity 1 to 3.5 ± 0.09% in parity 5. Lactose content remained relatively stable across parities, ranging from 4.7 ± 0.07% to 4.9 ± 0.08%. Analysis of variance (ANOVA) indicated no statistically significant differences (p > 0.05) in milk yield or composition across parity groups, though weak correlations suggested biologically relevant trends. Mid-parity stages (parities 3 and 4) were associated with optimal milk yield and compositional stability. Notably, the consistent lactose and protein levels in mid-parity milk may favor the enzymatic activation of the Lactoperoxidase (LP) system, offering potential benefits for natural milk preservation, particularly in decentralized or resource-limited places

Murrah buffalo, parity, milk yield, milk composition, Lactoperoxidase system, Dairy farming

Though parity did not show statistically significant effects on milk yield and key composition traits, weak correlations and trends provide practical insights. Regular parity-based assessments can enhance productivity and milk quality management in dairy herds. Monitoring parity can support more precise feeding and culling decisions. Parities 3 and 4 showed a balance between yield and quality, implying this stage as optimal for peak production

Alka Parmar and Vivek Sharma (2025). Effect of Parity on Milk Yield and Composition Traits in Murrah Buffaloes. Biological Forum, 17(7): 131-134