INTRODUCTION Soursop/Lakshman Phal (Annona muricata) belongs to the family Annonaceae, having diploid chromosome number 2n=14. It is wide spread in the tropics and frost free subtropics of the world (Samson, 1980). The soursop plant is cultivated mainly in home gardens. The eachtree yields up to 10 tons/ha with average each fruit weighs 0.5 to 2 kg. The fruit is compound in and covered with reticulated, leathery appearing but tender, inedible bitter skin from which protrude few or many stubby, or more elongated and curved soft, pliable “spines”. The skin is dark-green in the immature fruit, becoming slightly yellowish-green before the mature fruit is soft to the touch. In aroma, the pulp is somewhat pineapple-like, but its musky, subacid to acid flavor is unique (Schultes and Raffauf 1990). It is indigenous to most of the warmest tropical areas in South and North America including Amazon, it has become naturalized in many countries, and now has a wide distribution throughout tropical and subtropical parts of the world. The fruit makes an excellent drink or ice cream after straining. All parts of the tree are used in natural medicine in the tropics including the bark, leaves, root and fruit-seeds. The crushed seeds are used as an anthelmintic against internal and external parasitic worms. The bark leaves and roots are considered sedative, antispasmodic, hypoglycemic, hypotensive, smooth muscle relaxant and nervine and a tea is made for various disorders for those purposes (Holdsworth, 1990). Many bioactive compounds and phytochemicals have been found in plants and its various uses in natural medicine have been scientifically validated by many researchers (Heinrich, 1992; Sundarrao, 1993). Generally, the fruit and fruit juice is taken for worms and parasites, to cool fevers, to increase mother’s milk after childbirth (lactagogue), and as an astringent for diarrhea and dysentery. Soursop contains antibacterial, antiviral and antifungal properties. So this is a good source of medicine the juice of ripe fruit is said to be a diuretic, while a decoction of powdered immature fruits is used for dysentery remedy. In India, the average productivity of soursop is roughly 25-40 kg per plant. Soursop is only grown in small scale in the southern Indian states of Tamil Nadu, Karnataka, Andhra Pradesh, and Kerala. Soursop has a high economic value, it is widely cultivated and consumed as edible food. White fibrous juicy segments surround an extended receptacle in the fruit pulp. It could be used as a raw material for purees, juices, jams, jellies, powdered fruit bars, and flakes. The pulp can be used to make nectar (Peters et al., 2001). Soursop contains antibacterial, antiviral and antifungal properties. So this is a good source of medicine. The juice of ripe fruit is said to be a diuretic, while a decoction of powdered immature fruits is used for dysentery remedy. MATERIAL AND METHODS The present study on effect of humic acid, vermiwash and biofertilizer on growth and establishment of soursop was conducted under open condition during 2020-21 at Fruit Science Dept. College of Horticulture, Bengaluru. Seedlings were raised in polythene bags of 12 × 24 cm, then single seed was sown into every polybags containing media mixtures of Soil, FYM (1:1) ratio treated with humic acid, vermiwash and biofertilizers per treatment and these bags were kept under open condition. The design RBD was used for the experiment, there were twelve treatments with three replications. RESULT AND DISCUSSION The results showed that the inoculation of combination of humic acid, vermiwash and three bio-fertilizers; Azospirillum, spp. Pseudomonas fluorescence and AM fungi had produced significantly highest seedling height (39.00 cm), seedling girth (5.98 mm), number of leaves (25.20 no.), leaf area (49.00 cm2), fresh and dry Weight of shoot (30.66 and 8.10 g respectively) in the treatment T12: [Humic Acid (3%) + Vermiwash (1%) + Azospirillum spp ( 2g / seedling) + Pseudomonas fluorescence (2g / seedling) + AM Fungi (2g / seedling)]. And the maximum fresh and dry weight of root (19.40 and 2.80 g), number of primary roots (33.00), length of primary root (25.00 cm) and root volume (5.20 cm3) was also recorded in the treatment T12: [Humic Acid (3%) + Vermiwash (1%) + Azospirillum spp (2g / seedling) + Pseudomonas fluorescence (2g / seedling) + AM Fungi (2g / seedling)] at the end of 120 days in nursery.