Effects of Imidacloprid and Pendimethalin on Carbohydrates, Lipid and Protein Constituents of Pheretima posthuma

Author: Sakshi, Dharambir Singh* and Parveen Gill

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Abstract

The study was carried out for estimating the effect of pesticides imidacloprid and pendimethalin on biochemical constituents of Pheretima posthuma exposed for 90 days. Earthworm samples were collected on 1st and 90th days of treatment with imidacloprid and pendimethalin. Thereafter, earthworm powder was made which was then used for estimation of carbohydrate by Standard Phenol Sulphuric method, crude lipid by Soxhlet Extraction method and crude protein by Micro-Kjelhdal method respectively. Significant dose dependent reduction in biomolecules compositions observed in pesticides treated earthworms and maintaining the proper environment for properly measuring the carbohydrates, lipids, and protein constituents of Pheretima posthuma estimation was extremely difficult due to the rapid variation of these earthworm biomolecules.The combination dose of imidacloprid and pendimethalin were found to be more toxic than individual exposure of both chemicals. The maximum decrease in carbohydrate (4

Keywords

Pheretima posthuma, Soxhlet Extraction, Standard Phenol Sulphuric method

Conclusion

This study showed the degrading effect of Imidacloprid and Pendimethalin on carbohydrate, lipid and protein constituent of Pheretima posthuma. Imidacloprid was found to be more harmful than Pendimethalin when applied individually. So information provides by results, will be useful in estimating the effect of pesticides on Pheretima posthuma.

References

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How to cite this article

Sakshi, Dharambir Singh and Parveen Gill (2022). Effects of Imidacloprid and Pendimethalin on Carbohydrates, Lipid and Protein Constituents of Pheretima posthuma. Biological Forum – An International Journal, 14(1): 367-370.