Efficacy of Fungal and Bacterial Bioagents against Macrophomina phaseolina under in vitro conditions

Author: A. Srinivas, G. Uma Devi, B. Vidyasagar, P. Swarna Sree, M. Balram and B. Mallaiah

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Abstract

Charcoal rot disease of maize caused by Macroph¬omina phaseolina (Tassi) Goid reported to be majorly responsible for increased yield losses in the regions of arid and semi arid. It is difficult to manage charcoal rot as it is soil-borne in nature. Native fungal (Trichoderma harzianum) and bacterial (Bacillus spp., Pseudomonas spp. and Streptomyces spp.) bioagents were isolated from rhizosphere soil samples, collected from maize fields and they were tested for their efficacy to inhibit the mycelial growth of Macrophomina phaseolina in vitro. The Trichoderma harzianum isolate, MRTh-4 was found to be the most effective with 70.00% mycelial growth inhibition, where bacterial antagonists could able to inhibit in the range of 2.59% - 16.67% only. In conclusion, bacterial antagonists were inferior compared to Trichoderma harzianum in inhibiting the growth of the test pathogen and T. harzianum can become an important component in integrated disease management to achieve proper charcoal rot di

Keywords

Charcoal rot of maize, Macroph¬omina phaseolina, Trichoderma harzianum, Bacterial bioagents, Dual culture.

Conclusion

Trichoderma harzianum isolate, MRTh-4 was found to be the most effective with 70.00% mycelial growth inhibition. Bacterial antagonists were inferior compared to T. harzianum in inhibiting the growth of the test pathogen. It concludes that, T. harzianum can become an important component in integrated disease management to achieve proper charcoal rot disease management in the field.

References

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How to cite this article

A. Srinivas, G. Uma Devi, B. Vidyasagar, P. Swarna Sree, M. Balram and B. Mallaiah (2022). Efficacy of Fungal and Bacterial Bioagents Against Macrophomina phaseolina under in vitro Conditions. Biological Forum – An International Journal, 14(1): 317-322.