Estimation of Combining Ability using Line × Tester Analysis for Yield and its Contributing Traits in Aromatic rice (Oryza sativa L.)

Author: Khemlata Thakur, Abhinav Sao, Deepak Gauraha, Sunil Nair and Krishna Tandekar

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Abstract

The current investigation was conducted to study the estimates of combining ability and heterosis in order to comprehend the nature and kind of gene action, select superior genotypes, and generate the best cross combinations. Combining ability study for eleven yield characteristics was carried out on 25 crosses generated by utilising Line × Tester mating design. with 5 traditional non-aromatic lines including PKV HMT, RFS-2019-3, Zincorice MS, HMT Sona and Improved Samba Mahsuri and 5 aromatic testers including Pusa1121, Badshahbhog Sel-1, Trombay Chhattisgarh Dubraj Mutant (TCDM-1), Chhattisgarh Devbhog and R2281-308-1-185-1. ANOVA for Line × Tester design revealed that there was significant variance in treatments for all eleven characters studied. For all characters except panicle length, the variation due to line × tester effects were noted as significant. For all traits studied, the magnitude of Specific combining ability (SCA) variance was found to be higher than the General combining ability (GCA) variance, with the exception of days to 50% flowering, plant height (cm), panicle length (cm), and biological yield per plant (g), indicating a significant role of non-additive gene action (dominance & epistasis) in controlling these traits. The genotype PKV HMT among the lines and Pusa 1121 among the testers were found to be the best general combiners for grain yield per plant (g). Among the crosses the best specific combiner for grain yield per plant (g) observed for PKV HMT/Pusa 1121, Improved Samba Mahsuri/R2281-308-1-185-1, and Improved Samba Mahsuri/Chhattisgarh Devbhog, so, these hybrids can be utilized either for heterosis breeding or multiple cross-breeding programmes to acquire transgressive segregants and a large genetic base population for the improvement of quality rice production.

Keywords

Gene action, Non- additive, Specific combining ability, General combining ability, Heterosis

Conclusion

From the current study it is observed that the highest positive significant SCA effects for grain yield per plant (g) were recorded for cross PKV HMT/Pusa 1121, Improved Samba Mahsuri/R2281-308-1-185-1 and Improved Samba Mahsuri/Chhattisgarh Devbhog. This indicate there is involvement of non-additive gene action and they are effective combinations for creating high-yielding cultivars. The crosses PKV HMT/Trombay Chhattisgarh Dubraj Mutant (TCDM-1), PKV HMT/ Badshahbhog Sel-1, and Zinco Rice MS/Pusa 1121 showed the highest negative significant specific combining ability effects for days to 50% flowering, this indicates that there is a preponderance of additive gene action, so progeny selection will be effective for genetic improvement of this trait, negative values direct that these are good combiners and indicated the efficacy of these crosses in breeding for earliness. The maximum negative significant SCA effects have shown by cross HMT Sona/Pusa 112 followed by RFS-2019-3/ Badshahbhog Sel-1 and Zinco Rice MS/ Badshahbhog Sel-1 for plant height (cm) represented in Table 5. These crosses were used to develop dwarf and semi dwarf varieties.

References

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How to cite this article

Khemlata Thakur, Abhinav Sao, Deepak Gauraha, Sunil Nair and Krishna Tandekar (2023). Estimation of Combining Ability using Line × Tester Analysis for Yield and its Contributing Traits in Aromatic rice (Oryza sativa L.). Biological Forum – An International Journal, 15(7): 01-09.