Evaluation of Antagonistic Activity of Phylloplane Bacteria Against Finger Millet Blast Disease caused by Magnaporthe grisea

Author: G. Sobanbabu*, M. Theradimani, S. Thiruvudainambi, C Vannirajan, S. Rajavel, K. Kalpana, S. Sindhu and S. Brindhadevi

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Abstract

Finger millet (Eleusine coracana) is the most nutritious crop and it has severely affected by blast disease is caused by Magnaporthe grisea. The disease is effectively managed by fungicides and some resistant varieties. The resistant varieties are affecting by pathogen due to agroclimatic changes and the fungicides are hazardous to ecosystem. Biocontrol agent is an alternative method for managing the blast disease and it involve directly or indirectly inhibits the growth of the pathogen. Totally thirty bacterial isolates were isolated on phylloplane region of finger millet crop. Out of thirty bacterial isolates, four isolates were found to be effective antagonists viz., DPB1, KB1, KP1 and DPP1. Among them, KB1 isolate showed the maximum inhibition of mycelial growth of M. grisea (62.87%) followed by DPB1 isolate (54.54 %). On biochemical screening, Bacillus spp. were identified by gram staining, starch hydrolysis and protease production tests and Pseudomonas spp. were identified by gra

Keywords

Finger millet, Blast, Antagonists, Biochemical, Molecular characterization.