Evaluation of Bio-Agents against Rhizoctonia solani of Rice under in vitro conditions

Author: Goskula Kiran and Raghunath Mandal

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Abstract

Numerous biotic and abiotic factors have an impact on rice production and productivity, resulting in yield losses of up to 20–30%. considered In all regions of the world where rice is farmed, fungi infections are the most common biotic limitations. One of the main biotic restrictions on rice production in India is sheath blight, which is also the most economically significant disease of rice in the world. R. solani Kuhn, a fungal pathogen of both rice and soybeans, is the disease's cause (teleomorph: Thanatophorus cucumeris (Frank) Donk). When growing in a certain kind of naturally suppressive soil, a fungus called Trichoderma spp. protects plants from infectious diseases spread by pathogens in the soil. One of these soil-borne pathogens that severely damages economically significant crops is the fungus Rhizoctonia solani. In agriculture, using bio-control agents like Trichoderma spp. to fight infections and provide disease control is a viable option. The challenges regarding the bio-control of Rhizoctonia solani is scarely known to the farmers. Bio-control agents significantly suppress the growth of plant pathogenic microorganisms and regulate the rate of plant growth. The results of the present study suggest that Tricoderma harzianum has a highly antagonistic potential against the test pathogen. The experiment conducted to examine the responses of different isolates towards four bio control viz., T. viride, T. harzianum, Bacillus spp, Pseudomonas spp, by dual culture method. The zone inhibition of R. solani isolates in all four bio-agents was assessed. The findings clearly show that Tricoderma viride and Bacillus spp were the most efficient at inhibiting Rhizoctonia solani isolates. Trichoderma viride inhibited the growth of RS-10 by 77.1 percent. Bacillus spp. inhibited isolate no RS-5's growth the most.

Keywords

Rhizoctonia solani, Tricoderma spp, Bacillus spp

Conclusion

The pathogen R. solani significantly reduces the yield of important crops for the economy. T.viride, and Bacillus species, is extremely helpful in controlling diseases brought on by R. solani. Significant progress has been made as a result of the active marketing of T. viride and members of the Bacillus spp. family as bio-control agents. Because of the negative effects that pesticide residues have on the environment and the health of organisms, the market demand for bio-control agents and PGBR has increased recently. Bacillus species and R. solani's effects on the microbial community and the plant rhizosphere are being studied using metagenomic sequencing. A thorough understanding of the mechanism at work needs to be investigated despite the abundance of reports on the genetic understandings of the interactions between Bacillus species and R. solani and plants. In conclusion, Bacillus spp. and T. viride has multifaceted beneficial features that may be ideal for its integrated use in disease control. Various isolates of R. solani were successfully inhibited from growing in dual culture studies using the bioagents T. viride and Bacillus spp. The highest growth inhibition was observed against RS-5 in Bacillus species (68.9%), while the highest growth inhibition was observed against RS-3 in T. viride (77.10%).

References

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How to cite this article

Goskula Kiran and Raghunath Mandal (2023). Evaluation of Bio-Agents against Rhizoctonia solani of Rice under in vitro conditions. Biological Forum – An International Journal, 15(8a): 35-40.