Evaluation of Dolichos bean (Lablab purpureus var. typicus) germplasm for yield and quality

Author: Harini M., Ramar A.*, Janavi G. J. and Madhan Mohan M.

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Abstract

The current experiment was conducted at HC & RI Periyakulam in the summer of 2022 to assess the Dolichos bean's heritability, genetic variability, and genetic advance for yield, and yield-related characteristics. Due to dolichos bean genotype genetic variability and the possibility that it could be employed as parent material, it will be essential to evaluate its potential in order to put into action future crop enhancement programs. 26 genotypes of the bush-type Dolichos bean were investigated for 10 morphometric characteristics using a randomized block design with three replications. The genotype PKM LP 26 had the highest germination percentage (91.98%), followed by genotype PKM LP 11 (90.40%) and genotype PKM LP 06 (75.50%), which had the lowest germination percentage. The average plant height ranged from 44.89 -76.75 cm. The genotype PKM LP 04 (44.89 cm) recorded the minimum plant height and the genotype PKM LP 02 (76.75 cm) followed by PKM LP 27 (75.95 cm) recorded the maximum plant height. Earlier flowering was found in the genotype PKM LP 06 (38.30 days) while maximum days for first flowering was found in the genotype PKM LP 35 (53.45 days). Days to 50 % flowering ranged from 45.20-58.83 days with a mean value of 53.05 days. Genotype PKM LP 02 (6.93) recorded the highest no. of raceme plant-1 followed by the genotype PKM LP 11 (6.53) and the lowest no. of raceme plant-1 was found in the genotype PKM LP 19 (3.80). The highest no. of pod plant-1 was identified in the genotype PKM LP 26 (39.66) followed by the genotype PKM LP 11(38.45) and the genotype PKM LP 15 (24.18) recorded the lowest number of pod plant-1. Among all the genotype yield/plant was highest in the genotype PKM LP 26 (143.56 g) followed by the genotype PKM LP 11 (138.94 g). Both the number of pods produced by plant-1 and the yield of pods produced by plant-1 had high PCV and GCV, implying that these characteristics had greater magnitudes of variability and, as a result, more potential for development through selection. Except for germination percentage, initial flowering days, and 50% flowering days, all traits had strong heritability together with high genetic advance expressed as a percentage of the mean. The findings indicate that these features are influenced by additive gene action, therefore judicious choice based on all these phenotypical traits might be more effective.

Keywords

Dolichos bean, GCV, PCV, GAM and heritability

Conclusion

PKM LP 26 followed by PKM LP 11 had the maximum green pod plant-1 and the highest green pod yield per plant, respectively, @ 5 percent CD value. Hence, it can be concluded that PKM LP 26 is the best performance among taken up for trail. In the conclusion based on the estimations of genetic variability, individual plants could be carried out selection for characters viz., Pod/plant, pod yield/plant, and pod weight as they recorded high values of heritability and genetic advance.

References

INTRODUCTION One of the most popular native legume vegetables is dolichos bean (Lablab purpureus var. typicus), which is grown for its soft green pods. It is a perennial plant with bushy, upright, or climbing habits, yet it is planted as an annual. Its cultivation is influenced significantly by regional preferences. South India prefers green pods, while eastern India prefers white pods and northern India prefers green fleshy pods. It's a multi-purpose crop that can be eaten as a pulse, a vegetable, or fodder. The Dolichos lablab bean has a diverse set of qualities that can be used in a range of situations. One of its first advantages is its adaptability. It is drought tolerant and can thrive in a wide range of conditions. Dolichos beans exist in pole and bush varieties, each with its growth characteristics. Dolichos beans are an excellent source of protein, minerals, and vitamins (Golani et al., 2007). Due to their medicinal properties, lentils are employed in both traditional and modern healthcare systems (Morris 2009). The pole and bush forms of the dolichos bean come in a very broad variety of plant and pod features due to its Indian heritage. The dolichos bean has proven challenging to raise because of its low production, photosensitive nature, unpredictable growth habit, flowering habit, and consumer trends based on color, size, pod form, and aroma (Mishra et al., 2019). Breeding for productivity and the characteristics that contribute to it for any crop is influenced by polygene, environmental factors, and the level and type of genetic variability. PCV and GCV, which together reveal relative variation in different characteristics, which used to assess the level of variability. Therefore, to obtain an overall and accurate perspective, a quantitative assessment of the yield and its components is essential. The assessment of heritability examines how effectively a characteristic is passed down from one generation to the next generation. According to Chauhan et al. (2021) variation occurs between genotypes both chemo-typically and morphologically this variation may be due to its genetic nature. Low-degree environmental interactions affect highly heritable features related to yield, and traits related to yield in the selection program. Though genetic advances aid in the development of efficient selection techniques, heritability indicates the amount of quantitative trait inheritance. Genetic advances and heritability analyses enhance selection. The objective of the recent study was to assess the genetic advance, heritability, and genetic variability for several attributes among 26 Dolichos bean genotypes. MATERIALS AND METHODS During the year from 2021 to 2022, the experimental study was conducted at the Horticultural College and Research Institute in Periyakulam, Tamil Nadu, India, at the Western Block (10.13°N latitude, 77.59°E longitude) with an average altitude is 356 meters above sea level (Table 1). The experimental material comprised 26 genotypes of crops taken from various parts of Tamil Nadu and Kerala. During the summer, the selected genotypes were sowed in RBD with three replications. Each genotype was planted with a 60 × 30 cm spacing. To record observation and biometrical parameters as well as quality traits, five plants were chosen from each genotype and replication. Throughout the crop period, standard agronomic practices and prescribed plant protection measures were followed. From randomly selected five plants from each genotype, morphological observations such as plant type, pod color, seed color, and flower color were recorded. The genotype was characterized according to the documented descriptors. Five plants were chosen randomly from the total area of the plot, tagged, and observation was recorded growth, yield, and flowering characteristics were recorded at various stages: germination percentage, earlier flowering, days to 50% flowering, and the racemes/plant. Among the yield parameters were days taken for pod formation, number of pods per plant, pod length (cm), pod breadth (cm), number of seeds per pod, and pod yield per plant (Kg). By using (Panse & Sukhatme 1954) approach, analysis of variance was employed as a strategy to separate overall variation from those resulting from treatment and replication. The genotypic and phenotypic variance was calculated using the Burton method (1953). The method provided by Johnson et al. (1955) allowed for the calculation of genetic advance as a percentage of the mean and heritability. The Dolichos bean's 26 genotypes differed considerably in 4 qualitative parameters. The bloom colors range from white to pink to purple; all genotypes have a bushy growth habit. Most pods were green or light green. Each genotype pod can be consumed whole as a green vegetable. The seed was either white, brown, or black. All ten yield and yield-attributing characteristics under evaluation progressively significant variation in the proposed study's analysis of variance (ANOVA) (Table 3). Based on the mean performance the genotype PKM LP 26 (91.98%) was recorded with a maximum germination percentage followed by the genotype PKM LP 11 (90.40 %) and the genotype PKM LP 06 (75.50 %) recorded the minimum germination percentage. The average plant height ranged from 44.89 -76.75 cm (Table 2). The genotype PKM LP 04 (44.89 cm) recorded the minimum plant height and the genotype PKM LP 02 (76.75 cm) followed by PKM LP 27 (75.95 cm) recorded the maximum plant height. Earlier flowering was found in the genotype PKM LP 06 (38.30 days) while maximum days for first flowering was found in the genotype PKM LP 35 (53.45 days). Days to 50 % flowering ranged from 45.20-58.83 days with a mean value of 53.05 days. Genotype PKM LP 02 (6.93) recorded the highest number of raceme plant-1 followed by the genotype PKM LP 11 (6.53) and the lowest number of raceme plant-1 was found in the genotype PKM LP 19 (3.80). The highest number of pod plant-1 was identified in the genotype PKM LP 26 (39.66) followed by the genotype PKM LP 11(38.45) and the genotype PKM LP 15 (24.18) recorded the least number of pod plant-1. The genotype PKM LP 11(8.56cm) recorded the maximum pod length and minimum pod length was observed in the genotype PKM LP 15 (5.86cm). The genotype PKM LP 02 (1.68 cm) recorded the maximum pod width and the minimum pod width was recorded in the genotype PKM LP 19 (1.06 cm). The genotype PKM LP 08 (4.65) identified the maximum no of seeds per pod and the minimum seed /pod was recorded in the genotype PKM LP 01 (3.19). Among all the genotype yield/plant was highest in the genotype PKM LP 26 (143.56 g) followed by the genotype PKM LP 11 (138.94g). Genetic variability, heritability, and genetic advance Table 4 displays the heritability values, and estimated GAM, mean, range, GCV, and PCV (genotypic and phenotypic coefficients of variation) for the characteristics under investigation. A small difference between the GCV and PCV values for the characteristics suggests that they have had a high amount of genetic variability and, as a result, have a better chance of being selected. These characteristics show considerable genetic variability and are less affected by the environment. Table 3 presents the estimations of various genetic parameters. The yield and number of pods produced by plant-1 both showed high GCV and PCV, indicating that these characteristics had a greater order of magnitude of variability and, as a result, more opportunity for improvement through selection. Singh et al. (2015) verified the prior findings for the pod plant-1 and the plant yield in 24 genotypes of the dolichos bean. High PCV and GCV for green pod yield/plant were reported by Chattopadhyay & Dutta (2010) and Chaitanya et al. (2014), respectively. Moderate PCV and GCV are indicated by the plant height, racemes/ plant, pod length (cm), number of seeds/pod, and green pod width. This suggests that both additive and non-additive gene activity may contribute to the reported traits. These results were confirmed using the findings of Chaitanya et al. (2014), Hadavani et al. (2018), and Sahu & Bahadur (2018) for a number of seeds /pod in the Dolichos bean. For germination percentage, early flowering, and days to 50% flowering, PCV and GCV were low. These findings were supported by Savithiri et al. (2018) and Chaitanya et al. (2014), respectively. Apart from germination percentage, days for 50% flowering, and early flowering, the current study revealed significant heritability and high genetic progress as a percentage of the mean. According to the findings, these traits are influenced by additive gene action; as a result, careful choice based on such morphological parameters will be more effective. In terms of pod width, pod length, and the number of seeds per pod, the current findings were equivalent to those of Savitha (2008) and Rai et al. (2008). The results for pod width (cm), pod weight (g), and pod length (cm) in Dolichos bean genotypes were identical, based on Magalingam et al. (2013). Kujur et al. (2017) showed similar results for early flowering, tender pod length, and earlier flowering and pod width.

How to cite this article

Harini M., Ramar A.*, Janavi G. J., Madhan Mohan M. (2022). Evaluation of Dolichos bean (Lablab purpureus var. typicus) germplasm for yield and quality. Biological Forum – An International Journal, 14(3): 129-134.