Explore Pyocyanin Pigment extracted from Pseudomonas spp as Plant Growth Promoter

Author: Leena Dhake and Nutan Rathod

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Abstract

Agriculture is a source of income for over 55% of India's population. Chemical fertilizer and pesticides are used by farmers to increase productivity and profits. Increased use of chemical fertilizers and pesticides results in recalcitrant problems and harm to the texture of the soil. The use of biological agents to boost productivity and manage pests has been extensively researched as a solution. When utilized as bio-fertilizers or plant growth promoters, biological agents had stability and soil condition issues that prevented them from producing the best results. The most prevalent bacteria in the rhizosphere are Pseudomonas spp. It produces several metabolites in the soil, one of which is pyocyanin. It is a water-soluble blue-green phenazine pigment. In present studies Pseudomonas spp was isolated from a petrol pump soil sample near Jalgaon city. Pyocyanin pigment was extracted from the isolated organism using chloroform and HCl System. Pycocyanin's potential as a plant growth stimulant has been investigated. Extracted pigment was analysed and confirmed by UV visible Spectrophotometer, FTIR and HPLC. In UV- Visible spectrophotometer analysis absorption was observed at 520nm. FTIR analysis revealed different functional groups (-OH, C=N, -CH3, etc.) which belong to the aromatic structure of pyocyanin. HPLC analysis revealed three peaks with retention durations of 4.572, 5.009, and 5.295 with acetonitrile as mobile phase.

Keywords

Pyocyanin, Phenazine, pigment, Pseudomonas, plant growth promoter

Conclusion

The current study found that Pseudomonas spp. pigment is a naturally occurring material with the potential of function as a bio-fertilizer, contributing in accelerating the development of an environmentally friendly alternative to chemical fertilizers. For the benefit of society, its use as a fertiliser should be further investigated, along with toxicity testing.

References

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How to cite this article

Leena Dhake and Nutan Rathod (2023). Explore Pyocyanin Pigment extracted from Pseudomonas spp as Plant Growth Promoter. Biological Forum – An International Journal, 15(5): 981-987.