Fixation of Lethal Dose and Study of Physical and Chemical Effect of Mutagenesis on Germination, Pollen Sterility and Plant Survival in M1 Generation of Wheat (Triticum aestivum L.)

Author: Amit Rana, Vijay Rana, Priyanka*, Shubhanshu Anubhav and Chetan Gupta

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Abstract

The present study comprises of four wheat varieties viz., HS 490, HPW 89, HPW 360 and HPW 251 which were treated with 12 doses each of gamma rays viz., 25Gy, 50Gy, 75Gy, 100Gy, 125Gy, 150Gy, 175Gy, 200Gy, 225Gy, 250Gy, 275Gy and 300Gy and EMS concentrations viz., 0.05%, 0.15%, 0.25%, 0.35%, 0.45%, 0.55%, 0.65%, 0.75%, 0.85%, 0.95%, 1.05% and 1.15% to study their effect on germination, pollen fertility reduction and plant survival in M1 generation under both lab and field conditions. The results showed overall reduction in germination percent, plant survival and increased pollen sterility over control in all the mutagenic treatments. LD50 values observed using probit analysis of all four varieties ranged from 0.683%-1.169% EMS concentration and 241.41-278.48 Gy. Therefore, concentrations/dose of both mutagens producing less lethality would be suitable for determining varietal effect and inducing large scale mutagenesis in future wheat breeding programmes.

Keywords

Gamma rays, EMS, mutagen, germination, LD50, pollen grains, survival.

Conclusion

In the present study, effect of induced mutagenesis on germination percentage were observed in four wheat varieties using gamma rays and EMS mutagens. Irradiation with gamma dose at 300 Gy, HS 490 (63%) and HPW 251 (62%) showed highest reduction in germination over control whereas at 1.15% EMS treatment, HPW 251 (58%) and HPW 360 (55%) showed highest reduction in germination per cent over control indicating reduction in germination per cent in all the mutagenic treatments with increase in the concentration of the mutagens. Selection of appropriate mutagen and its dose plays a pivotal role in success of any mutation breeding programme. The LD50 value of mutagen helps in predicting mutagen sensitivity and subsequent recovery of higher mutation frequency. Hence, it is vital to optimize lethal dose of mutagens to isolate desired utilizable mutations (Hazra et al., 2021). LD50 values observed under EMS and gamma irradiation treatment for varieties viz., HS 490, HPW 89, HPW 360 and HPW 251 w

References

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How to cite this article

Rana, A.; Rana, V.; Priyanka; Anubhav, S. and Gupta, C. (2022). Fixation of Lethal Dose and Study of Physical and Chemical Effect of Mutagenesis on Germination, Pollen Sterility and Plant Survival in M1 Generation of Wheat (Triticum aestivum L.). Biologic