Gelatin Zymography of Matrix Metalloproteinases in Serum Samples of Kangayam Breed Cattle

Author: T.C. Balamurugan, V. Haripriya, R. Prakash Krupakaran, G. Anandhi, E.L. Aadhie Shrie, M. Vishal and M. Swathi

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Abstract

The current study was carried out to ascertain the demonstration of gelatinases viz., MMP-2 and MMP-9 in serum of Kangayam cattle, an indigenous cattle breed of Tamil Nadu. Experimental animals were divided into two groups viz., Group I Male (2-4 yr), Group II Female (2-4 yr). Gelatin zymography was done on all serum samples, and it was established that the chief bands were found at 220, 125, and 92 kDa of MMP-9 and 72 kDa of MMP-2 in both groups. Two noticeable bands were observed at 92 kDa and 72 kDa, which represent the pro-forms of MMP-9 and MMP-2, respectively. Additionally, two lytic bands were found in each group at 220, 125 kDa, which represent the proforms of MMP-9; similarly, the older groups displayed thicker bands at 135 and 220 kDa, which indicate the expression of proenzymatic forms of MMP-9. In order to determine the sex difference, the latent form of MMP-2 (72 kDa) was 1.5 times more intense in male groups than in female groups. Male groups had a lower level of latent form MMP-9 intensity compared to female groups. Comparably, two more noticeable bands at 220 and 125 kDa were observed in the male groups, showing the proenzymatic forms of MMP-9 and confirming that they are the lytic bands of MMP-9. It was determined that both groups had proven expression of gelatinase activity. However, male groups expressed more MMP-2 than female groups did, whereas female groups expressed more MMP-9 than male groups. Therefore, because MMPs are involved in the buildup of extracellular matrix and are linked to concentric remodeling of tissues, age-dependent changes in MMP profiles occurred as a function of age.

Keywords

Gelatin zymography, MMP-2, MMP-9 Kangayam cattle, Bands

Conclusion

It was determined that both sexes had proven expression of gelatinase activity. The expression of MMP-9 was higher in female groups than in male groups, while MMP-2 was higher in male groups than in female groups. Further, there is more up regulation of MMP-2 mediated through MMP-9 activity observed in Kangayam cattle serum. Because MMPs are involved in the formation of extracellular matrix and are linked to the concentric remodeling of tissues, age-dependent changes in MMP profiles were observed as a function of age.

References

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How to cite this article

T.C. Balamurugan, V. Haripriya, R. Prakash Krupakaran, G. Anandhi, E.L. Aadhie Shrie, M. Vishal and M. Swathi (2025). Gelatin Zymography of Matrix Metalloproteinases in Serum Samples of Kangayam Breed Cattle. Biological Forum, 17(2): 67-70.