Genetic Variability Studies in large Seeded Genotypes of Peanut (Arachis hypogaea L.)

Author: K. Deepthi*, K. John, D. Bharathi and P. Sudhakar

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Abstract

Genetic studies determining the valuable confectionary characters like oil, protein, sucrose and fatty acid contents, in addition to yield components like kernel weight, shelling percentage are limited. The quality requirement of large-seeded groundnut is more precise and distinctly different from peanut as an oilseed crop. Because of the above fact, there is a necessity to evaluate the available large- seeded groundnut genotypes to know the extent of genetic variability and heritability for important confectionery characteristics. Therefore, Genetic variability analysis was carried out for physiological, yield attributes and quality traits in 26 peanut genotypes. The study indicated the existence of a considerable amount of variability for every character. High heritability estimates were recorded for sucrose content, days to maturity, sound mature kernel per cent, days to 50 % flowering, stearic acid content, dry haulms yield plant-1, oil content, oleic to linoleic acid ratio, linoleic acid content, shelling per cent, kernel yield plant-1, plant height, protein content, pod yield plant-1, oleic acid content, specific leaf area at 45 DAS, palmitic acid content, harvest index and a number of mature pods plant-1 stipulating that the effect of environment is least in the countenance of these characters. Sucrose content, dry haulms yield plant-1, the number of mature pods plant-1, plant height, kernel yield plant-1, pod yield plant-1, stearic acid content, shelling per cent and harvest index exhibited high genetic advance as per cent of mean. High heritability coupled with high genetic advance as per cent of mean (GAM) was recorded for sucrose content, stearic acid content, dry haulms yield plant-1, shelling per cent, kernel yield plant-1, plant height, pod yield plant-1, harvest index and the number of mature pods plant-1 indicating a preponderance of additive gene action. Therefore, selection for the above characters would be more rewarding to bring improvement in peanut for physiological, yield attributes and quality traits.

Keywords

Peanut, Broad sense heritability, Genetic advance, GAM, Variability

Conclusion

The present study revealed a higher magnitude of variability for most of the yield and quality traits, which is also accompanied by high heritability and genetic advance. High heritability coupled with high genetic advance as per cent of mean was observed for sucrose content, stearic acid content, dry haulms yield plant-1, shelling per cent, kernel yield plant-1, plant height, pod yield plant-1, harvest index and the number of mature pods plant-1. This indicates that these characters were under additive genetic control and selection for genetic improvement will be worthwhile and may rapidly contribute to yield and quality improvement.

References

INTRODUCTION Cultivated peanut (Arachis hypogaea L.) is an annual legume crop. It is a self-pollinated crop with a chromosome number of 2n = 4x = 40. It is a native of South America and grown all over the tropical and sub-tropical regions of the world. Peanut belongs to the sub-family Papilionaceae of the family Leguminosae and it is commonly called a poor man’s nut. Peanut is widely accepted as the most critical source of nutrition for both humans and animals due to its high oil (45-50%) and protein (25%) content. Apart from oil and protein, peanut contains carbohydrates (8-14%), minerals, vitamins A, B and some members of the B2 group, mainly thiamin and niacin and are abundant in mono-unsaturated fatty acids. As peanut is an major food crop and recently the confectionary types has presumed great importance as snack food in national and international markets, there is a necessity to enhance the quality traits of peanut. Hence selection for quality attributes in peanut is a requisite in breeding programmes. However, genetic studies determining these valuable confectionary characters like oil, protein, sucrose and fatty acid contents, in addition to yield components like kernel weight, sound mature kernels and shelling percentage are limited. The breeding procedures involving confectionary genotypes are to be improved. The quality requirement of large-seeded groundnut is more precise and distinctly different from peanut as an oilseed crop. Because of the above fact, there is a necessity to evaluate the available large- seeded groundnut genotypes to know the extent of genetic variability and heritability for important confectionery characteristics. The magnitude of genetic variability can determine the pace and quantum of genetic improvement through selection or hybridization followed by selection. Hence, it is essential to study the genetic variability parameters like range, coefficient of variability, heritability and genetic advance over a mean (Parameshwarappa et al. 2005, Korannavar and Biradar 2019). The present investigation was taken up among twenty-six genotypes of peanut to assess the amount of genetic variability for physiological, yield attributes and quality traits in peanut. MATERIAL AND METHODS The present investigation was carried out among 26 peanut genotypes during Kharif, 2020, at a dry land farm of Sri Venkateswara Agricultural College, Tirupati using Randomized Block Design with three replications. Observations were noted on five randomly selected plants in each genotype for SPAD chlorophyll meter reading at 45 DAS, specific leaf area at 45 DAS, relative water content, plant height, number of primary branches plant-1, number of mature pods plant-1, hundred pod weight, shelling per cent, hundred kernel weight, kernel yield plant-1, sound mature kernel per cent, dry haulms yield plant-1, harvest index, oil content, protein content, sucrose content, palmitic acid content, stearic acid content, oleic acid content, linoleic acid content, oleic to linoleic acid ratio and pod yield plant-1 whereas for days to 50 % flowering and days to maturity observations were noted on a plot basis. The variation among 26 genotypes for different characters was tested for significance by using the analysis of variance technique as given by Panse and Sukhatme (1961). The genotypic (GCV) and phenotypic (PCV) coefficient of variation was calculated by the formulae given by Burton (1952). Heritability in broad sense [h2b] was calculated by the formula given by Lush (1940) and genetic advance as given by Johnson et al. (1955). RESULTS AND DISCUSSION The analysis of variance carried out for 24 physiological, yield attributes and quality traits revealed highly significant (1%) differences among the genotypes for the characters, viz., days to 50 % flowering, days to maturity, SPAD chlorophyll meter reading at 45 DAS, specific leaf area at 45 DAS, relative water content, plant height, number of primary branches plant-1, number of mature pods plant-1, hundred pod weight, shelling per cent, hundred kernel weight, kernel yield plant-1, sound mature kernel per cent, dry haulms yield plant-1, harvest index, oil content, protein content, sucrose content, palmitic acid content, stearic acid content, oleic acid content, linoleic acid content, oleic to linoleic acid ratio and pod yield plant-1 (Table 1). These results indicated the presence of a considerable amount of genetic variation for all the 24 traits in the experimental material. A. Variability studies The estimates of genetic parameters viz., phenotypic and genotypic coefficient of variation, heritability in broad sense, genetic advance and genetic advance as per cent of mean computed for twenty-four characters in twenty-six genotypes are presented in Table 2. The Phenotypic coefficient of variation was of high magnitude than the genotypic coefficient of variation for all the characters indicating the influence of the environment in the expression of these traits. These results are following the findings of Narasimhulu et al. (2012), John et al. (2013), Kamdi et al. (2017), Mahesh et al. (2018) and Nagaveni and Hasan Khan (2019). The characters, sucrose content, dry haulms yield plant-1, the number of mature pods plant-1 and plant height showed high variability indicating an adequate amount of variation among the genotypes. Therefore, simple selection would be productive for further improvement of these characters. Higher GCV and PCV estimates for plant height, number of mature pods plant-1 and dry haulms yield plant-1 were following the results obtained by Kadam et al. (2016) and Mahesh et al. (2018). High estimates of GCV for sucrose content were similar to the reports of Rathod and Toprope (2018). The characters, specific leaf area at 45 DAS, sound mature kernel per cent, days to 50 % flowering, days to maturity, oleic acid content, protein content, linoleic acid content, palmitic acid content, SPAD chlorophyll meter reading at 45 DAS, relative water content and oil content exhibited lower estimates of both PCV and GCV. Lower estimates of GCV and PCV for days to 50 % flowering were by the results of Vasanthi et al. (2015), Kadam et al. (2016), Chavadhari et al. (2017), GonyaNayak et al. (2018) and Mahesh et al. (2018). The low variability estimates recorded for SPAD chlorophyll meter reading at 45 DAS and days to maturity were confirmed with the findings of Rathod and Toprope (2018) and Gonya Nayak et al. (2018). Jayalakshmi et al. (2003) showed similar results for specific leaf area at 45 DAS. Lower estimates of GCV and PCV for protein content and oil content were confirmed with the findings of Vasanthi et al. (2015), Omprakash and Nadaf (2017) and Mahesh et al. (2018), whereas low variability for relative water content and sound mature kernel per cent were similar with the findings of Nagaveni and Hasan Khan (2019). B. Heritability High heritability estimates were recorded for sucrose content, days to maturity, sound mature kernel per cent, days to 50% flowering, stearic acid content, dry haulms yield plant-1, oil content, oleic to linoleic acid ratio, linoleic acid content, shelling per cent, kernel yield plant-1, plant height, protein content, pod yield plant-1, oleic acid content, specific leaf area at 45 DAS, palmitic acid content, harvest index and the number of mature pods plant-1. The characters viz., hundred pod weight and hundred kernel weight exhibited moderate heritability. The characters, the number of primary branches plant-1, relative water content and SPAD chlorophyll meter reading at 45 DAS exhibited lower heritability estimates. C. Genetic advance High genetic advance was exhibited for the character’s specific leaf area at 45 DAS and hundred pod weight, whereas dry haulms yield plant-1, plant height, shelling per cent, days to maturity, sound mature kernel per cent and harvest index recorded moderate genetic advance. The characters viz., hundred kernel weight, pod yield plant-1, number of mature pods plant-1, sucrose content, kernel yield plant-1, oleic acid content, linoleic acid content, days to 50% flowering, protein content, oil content, relative water content, SPAD chlorophyll meter reading at 45 DAS, palmitic acid content, stearic acid content, number of primary branches plant-1 and oleic to linoleic acid ratio recorded low genetic advance. Genetic advance as per cent of mean. Higher genetic advance as per cent of mean was recorded for sucrose content, dry haulms yield plant-1, the number of mature pods plant-1, plant height, kernel yield plant-1, pod yield plant-1, stearic acid content, shelling per cent and harvest index. The characters exhibited a moderate genetic advance as per cent of mean, oleic to linoleic acid ratio, sound mature kernel per cent, hundred pod weight, days to 50% flowering, hundred kernel weight, days to maturity and specific leaf area at 45 DAS. The characters viz., the number of primary branches plant-1, linoleic acid content, oleic acid content, protein content, palmitic acid content, oil content, SPAD chlorophyll meter reading at 45 DAS and relative water content exhibited low genetic advance as per cent of mean. High heritability coupled with high genetic advance as per cent of mean was recorded for sucrose content, stearic acid content, dry haulms yield plant-1, shelling per cent, kernel yield plant-1, plant height, pod yield plant-1, harvest index and number of mature pods plant-1 indicating preponderance of additive gene action and hence phenotypic selection would be more effective for improvement of these characters. High heritability coupled with high genetic advance as per cent of mean for number of mature pods plant-1 was also reported by Chavadhari et al. (2017) and Mahesh et al. (2018). Similar results for plant height were reported by Rao et al. (2014) and Yusuf et al. (2017). Narasimhulu et al. (2012) reported similar results for shelling per cent. High heritability coupled with high genetic advance as per cent of mean for kernel yield plant-1 and pod yield plant-1 were similar to the findings of Rao et al. (2014), Gupta et al. (2015), Rajarathinam et al. (2017) and Bugati et al. (2020). Mahesh et al. (2018) and Nagaveni and Hasan Khan (2019) reported similar results for dry haulms yield plant-1. High heritability coupled with high genetic advance as per cent of mean for harvest index are by the reports of Vasanthi et al. (2015) and Chavadhari et al. (2017). Yusuf et al. (2017) reported similar results for stearic acid content. High heritability coupled with high genetic advance as per cent of mean for sucrose content conforms to the reports of Rathod and Toprope (2018). High heritability coupled with moderate genetic advance as per cent of mean was exhibited by characters viz., days to maturity, sound mature kernel per cent, days to 50 % flowering, oleic to linoleic acid ratio and specific leaf area at 45 DAS. This finding obviates simple selection and necessitates recombination breeding with a postponement of selection at later generations. High heritability coupled with moderate genetic advance as per cent of mean for sound mature kernel per cent were similar to the reports of Kumar et al. (2019). Similar results were reported for days to 50 % flowering by John and Reddy (2014). Characters viz., oil content, linoleic acid content, protein content, oleic acid content and palmitic acid content recorded high heritability coupled with low genetic advance as per cent of mean, which indicated the presence of non-additive gene action. High heritability is exhibited due to the favourable influence of environment rather than genotype and hence selection for such traits may not be rewarding. High heritability coupled with low genetic advance as per cent of mean for protein and oil content were also reported by Kumar et al. (2019). Moderate heritability and moderate genetic advance as per cent of mean were recorded for the characters, hundred pod weight and hundred kernel weight, indicating that both additive and non-additive gene actions have a role in inheritance and phenotypic selection would be adequate to some extent. Similar kinds of results for moderate heritability coupled with moderate genetic advance as per cent of mean for hundred pod weight and hundred kernel weight was reported by Patil et al. (2014) and Shinde et al. (2019). Low heritability and low genetic advance as per cent of mean were recorded for characters viz., then umber of primary branches plant-1, relative water content and SPAD chlorophyll meter reading at 45 DAS, which indicates that these characters were highly influenced by environmental effects and selection would be ineffective. Low heritability and low genetic advance as per cent of mean for SPAD chlorophyll meter reading at 45 DAS and relative water content were similar to the findings of Srivalli and Nadaf (2016).

How to cite this article

K. Deepthi*, K. John, D. Bharathi and P. Sudhakar (2022). Genetic Variability Studies in large Seeded Genotypes of Peanut (Arachis hypogaea L.). Biological Forum – An International Journal, 14(2): 1046-1051.