Histopathology and Immunohistochemistry Associated with Cutaneous Tumours in Canines

Author: Aditya Sharma and Sheikh Uzma Farooq

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Abstract

The present study was designed to characterize and diagnose different spontaneously occurring canine cutaneous neoplasms. As there are different ways to diagnose and identify the tumours, it becomes important to determine the methodology for tumour detection. Conventional methods for diagnosis of tumours usually gives the idea whether the tumour is benign or malignant, however advanced methods such as immunohistochemistry and molecular tissue markers have also gained popularity due to the minute details they provide regarding the tumours. Twenty-nine tumour samples were collected from dogs irrespective of their sex, breed, age and were grouped as skin tumours based upon their anatomical location. Histopathological diagnosis of tumour samples evinced 12 benign skin tumours (perianal gland adenoma-5, trichoblastoma-4, trichofolliculoma-1, tricholemmoma-1 and haemangioma-1) and 17 malignant skin tumours (fibrosarcoma-6, mast cell tumour-3, apocrine adenocarcinoma-3, haemangiopericytoma-1, perianal gland adenocarcinoma-1, chondrosarcoma-1, melanoma-1, poorly differentiated carcinoma-1). A total of 10 cutaneous tumours were subjected to immunohistochemistry for various molecular markers, viz., F8, SMA, Desmin, S-100, p63 and ER. Various tumour subtypes were found positive for different IHC markers.

Keywords

Cutaneous neoplasms, Histopathological, Benign, Malignant, Immunohistochemistry

Conclusion

It was deduced from the present investigations that advanced technique of immunohistochemistry alone, cannot aid in the proper grading, staging and characterization of tumours of the animal body. Although, it is comparatively easier to diagnose different benign tumours by cytology and histopathology but a combination of cytology, histopathology and immunohistochemistry are required for malignant tumours diagnosis. However molecular techniques are relatively very costly as compared to the conventional techniques of histopathology and cytology, hence its extensive use for veterinary purpose is still a challenge.

References

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How to cite this article

Aditya Sharma and Sheikh Uzma Farooq (2023). Histopathology and Immunohistochemistry Associated With Cutaneous Tumours in Canines. Biological Forum – An International Journal, 15(5): 1024-1030.