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Identification and Molecular characterization of Orf virus from Goats of Thanjavur District of Tamilnadu

Author: Manickam R., Puvarajan B., Balasubramaniam A., Balakrishnan S. and Selvaraj J.

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Abstract

Contagious pustular dermatitis in goats as often referred as Orf or Scabby mouth being a viral etiology is described to be highly contagious and causing economic losses to the animal husbandry sector. Thanjavur District of Tamilnadu being an agrarian background and depend mostly on livestock and orf like contagious disease pose a serious threat at times affecting their livelihood which has been addressed by earlier and rapid diagnosis .Twenty one number of Orf suspected cases from three flocks of goats in Thanjavur District (Cauvery Delta Region, Tamilnadu) exhibited skin lesions such as erythema and pustules with crusty areas over the muzzle region and perianal areas. The scab material was collected from severely affected goats showing characteristic symptoms and subjected to viral DNA extraction and subsequently the conserved B2L gene was amplified by polymerase chain reaction. The PCR test carried out showed positivity of B2L gene in nineteen samples confirming the Orf virus. As a result, the PCR technique was found to be a swift tool for diagnosis which proved to help in advocating measures for further curtailment of Orf among goats in Cauvery Delta Region of Tamilnadu

Keywords

Contagious ecthyma - Goat - Orf virus - B2L gene – PCR

Conclusion

From the present investigation it is concluded that the skin lesions in goat were caused by orf viruses (ORFV) and confirmed by molecular method such as Polymerase chain reaction and the challenges posed by sudden occurrence of orf in goats in Thanjavur region was controlled successfully by application of early interventions by separation and treatment of ailing animals and advocation of biosecurity measures in the farm.

References

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How to cite this article

Manickam R., Puvarajan B., Balasubramaniam A., Balakrishnan S. and Selvaraj J. (2023). Identification and Molecular characterization of Orf virus from Goats of Thanjavur District of Tamilnadu. Biological Forum – An International Journal, 15(8a): 348-351.