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Identification of True Rice Hybrids Carrying the Brown Planthopper Resistance Gene through Foreground Marker Analysis

Author: M. Tushara, B. Krishnaveni, K. Radhika, V. Roja, V. Jhansi Lakshmi and A.D.V.S.L.P. Anand Kumar

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Abstract

The brown planthopper is the most widespread and damaging pests of rice, causing yield losses of over 50 percent. This study targeted on the introgression of the brown planthopper resistant gene into a high-yielding variety using marker-assisted backcross breeding. In this context, the investigation aimed to identify F1 individuals possessing the BPH resistant gene Bph33(t) through linked markers. The F1 progeny was obtained by crossing two rice varieties BPT5204 (well known for slender grain and quality traits) and RP2068-18-3-5 (a BPH-resistant donor) having Bph33(t) gene. Among the three SSR markers validated, only heat shock protein gene marker exhibited polymorphism between the parents and was used further for foreground selection. Among the 18 tested F1 progenies, 15 were found to carry the Bph33(t) gene. The identified F1 progenies will be utilized in marker assisted backcross breeding programs aimed at enhancing BPH resistance in rice

Keywords

Rice, hybrids, brown plant hopper, resistance, foreground selection, markers

Conclusion

The study concludes that the heat shock protein gene(LOC_Os01g42190.1) effectively distinguished true F1 plants from non-hybrids. SSRs are highly reliable codominant markers, ideal for identifying true hybrids. The identified polymorphic marker can be utilized as a foreground marker for identifying plants with Bph33(t) gene in Marker-Assisted Selection (MAS) programs. F1 plants carrying the BPH resistance gene will be used in a Marker-Assisted Backcross Breeding (MABB) program to introgress the Bph33(t) gene from the donor parent, RP 2068 or selfed to produce an F2 generation. MAS accelerate and enhances the efficiency of gene introgression compared to conventional breeding methods

References

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How to cite this article

M. Tushara, B. Krishnaveni, K. Radhika, V. Roja, V. Jhansi Lakshmi and A.D.V.S.L.P. Anand Kumar (2024). Identification of True Rice Hybrids Carrying the Brown Planthopper Resistance Gene through Foreground Marker Analysis. Biological Forum – An International Journal, 16(10): 30-34.