Brassica juncea (L.) is economically important oilseed crop, next to groundnut, due to its high oil content and superior oil quality. Wounding caused due to agricultural practices and insect attack is the most common stress, resulting in extensive loss of vegetative tissues. To understand the mechanism of wound response in B. juncea wound treatment was given and samples were collected at three different time point intervals (control and wound samples). RNAs of all three-time points interval was pooled together and RNA sequencing was done. RNA sequencing gave transcriptome data, in B juncea 1503 genes were upregulated and 1769 genes were downregulated genes (DEGs). In this study transcriptome analysis of wounded B. juncea deciphered the upregulation of stress proteins including vegetative cell wall protein gp1-like, protein MLP1-like isoform X1, F-box/LRR-repeat protein 7, protein TIFY 10B, myb-like protein Xin wounded B. juncea compared to control B. juncea. Transcription factor bHLH92, bHLH149, bHLH74, WRKY transcription factor 6-like, WRKY transcription factor 18-like, 75, transcription factor MYB6-like and transcription factor PosF21, signalling kinases like mitogen-activated protein kinase ANP1-like, serine/threonine-protein kinase NAK, receptor like protein kinase S.2-like, glycerol kinase-like, serine/threonine-protein kinase Nek6, metabolite synthesizing enzymes cellulose synthase, galactinol-sucrose galactosyltransferase 2, callose synthase 5, flavonol synthase/flavanone 3-hydroxylase were upregulated in wounded B. juncea compared to control B. juncea. ROS scavenging enzymes, methionine sulfoxide reductase A4, glutathione S-transferase U24, peroxidase 71, L-ascorbate peroxidase 1, catalase were upregulated while superoxide dismutase catalase-2 and catalase-3 were down regulated in wounded B. juncea compared to control leaves. Upregulation of these significant genes suggests that they have potential role in wound stress recovery and these genes can be employed for transgenic crop development.

B. juncea, wound stress, Transcription factors, MAP kinases, ROS

The transcriptome analysis of the wounded B. juncea revealed the upregulation of genes encoding a wide array of proteins involved in signalling, defense, and metabolic processes. In response to wounding plant produces signals that rapidly activate MAPK. These MAPK signalling proteins could be activated by reactive oxygen species. MAPK and receptor kinases were found to be upregulated in the wound transcriptome. Wounding in plant results in the activation and transcription of genes coding for proteins involved in numerous cellular and molecular functions. Signalling kinases, stress-related proteins, transcription factors, metabolic enzymes and ROS scavenging enzymes together interplay vital role to recover damage caused by wounding.

Sandesh Waghmare and Anita Grover (2023). Identification of Wound Responsive Genes in Brassica juncea by Transcriptome Analysis. Biological Forum – An International Journal, 15(8): 466-469.